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Data from: Parallel proteomic and phosphoproteomic analyses of successive stages of maize leaf development

Citation

Facette, Michelle R. et al. (2013), Data from: Parallel proteomic and phosphoproteomic analyses of successive stages of maize leaf development, Dryad, Dataset, https://doi.org/10.5061/dryad.9qv2k

Abstract

We carried out large-scale, quantitative analyses of the maize (Zea mays) leaf proteome and phosphoproteome at four developmental stages. Exploiting the developmental gradient of maize leaves, we analyzed protein and phosphoprotein abundance as maize leaves transition from proliferative cell division to differentiation to cell expansion, and compared these developing zones to one another and the mature leaf blade. Comparison of the proteomes and phosphoproteomes suggests a key role for post-translational regulation in developmental transitions. Analysis of proteins with cell wall- and hormone-related functions illustrates the utility of the dataset and provides further insight into maize leaf development. We compare phosphorylation sites identified here to those previously identified in Arabidopsis. We also discuss instances where comparison of phosphorylated and unmodified peptides from a particular protein indicates tissue-specific phosphorylation. For example, comparison of unmodified and phosphorylated forms of PIN1 suggests a tissue-specific difference in phosphorylation, which correlates with changes in PIN1 polarization in epidermal cells during development. Together, our data provide insights into regulatory processes underlying maize leaf development, and provide a community resource cataloging the abundance and phosphorylation status of thousands of maize proteins at four leaf developmental stages.

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