Data from: Atlas of Plasmodium falciparum intraerythrocytic development using expansion microscopy
Data files
Sep 28, 2023 version files 288.44 GB
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(10).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(11).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(12).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(13).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(14).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(15).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(16).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(17).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(18).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(19).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(2).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(20).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(21).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(22).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(23).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(24).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(3).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(4).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(5).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(6).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(7).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(8).czi
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3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan_Processed_(9).czi
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3D7_NHS405_Aldolase488_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_Aldolase488_SytoxRed_Airyscan_Processed_(10).czi
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3D7_NHS405_Aldolase488_SytoxRed_Airyscan_Processed_(11).czi
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3D7_NHS405_Aldolase488_SytoxRed_Airyscan_Processed_(2).czi
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3D7_NHS405_Aldolase488_SytoxRed_Airyscan_Processed_(4).czi
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3D7_NHS405_Aldolase488_SytoxRed_Airyscan_Processed_(5).czi
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3D7_NHS405_Aldolase488_SytoxRed_Airyscan_Processed_(6).czi
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3D7_NHS405_Aldolase488_SytoxRed_Airyscan_Processed_(7).czi
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3D7_NHS405_Aldolase488_SytoxRed_Airyscan_Processed_(8).czi
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3D7_NHS405_Aldolase488_SytoxRed_Airyscan_Processed_(9).czi
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3D7_NHS405_AMA1_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(10).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(11).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(12).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(13).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(14).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(15).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(16).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(17).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(18).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(19).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(2).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(20).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(21).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(22).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(23).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(24).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(25).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(26).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(27).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(28).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(29).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(3).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(4).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(5).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(6).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(7).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(8).czi
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3D7_NHS405_BIP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(9).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(10).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(11).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(12).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(13).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(14).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(15).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(16).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(17).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(18).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(2).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(3).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(4).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(5).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(6).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(7).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(8).czi
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3D7_NHS405_BODIPYTRc_SytoxRed_Airyscan_Processed_(9).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(10).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(11).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(12).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(13).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(14).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(15).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(16).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(17).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(18).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(19).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(2).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(20).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(21).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(22).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(23).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(24).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(25).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(26).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(27).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(28).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(3).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(4).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(5).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(6).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(7).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(8).czi
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3D7_NHS405_Centrin(20H5)_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(9).czi
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3D7_NHS405_Centrin(20H5)_488_Tubulin594_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_Centrin(20H5)_488_Tubulin594_SytoxRed_Airyscan_Processed_(2).czi
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3D7_NHS405_Centrin(20H5)_488_Tubulin594_SytoxRed_Airyscan_Processed_(3).czi
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3D7_NHS405_Centrin(20H5)_488_Tubulin594_SytoxRed_Airyscan_Processed_(4).czi
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3D7_NHS405_Centrin(20H5)_488_Tubulin594_SytoxRed_Airyscan_Processed_(5).czi
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3D7_NHS405_Centrin(20H5)_488_Tubulin594_SytoxRed_Airyscan_Processed_(6).czi
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3D7_NHS405_Centrin(20H5)_488_Tubulin594_SytoxRed_Airyscan_Processed_(7).czi
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3D7_NHS405_EBA175_488_AMA1_555_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_EBA175_488_AMA1_555_SytoxRed_Airyscan_Processed_(2).czi
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3D7_NHS405_EBA175_488_AMA1_555_SytoxRed_Airyscan_Processed_(3).czi
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3D7_NHS405_EBA175_488_AMA1_555_SytoxRed_Airyscan_Processed_(4).czi
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3D7_NHS405_EBA175_488_AMA1_555_SytoxRed_Airyscan_Processed_(5).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(10).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(11).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(12).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(13).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(14).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(15).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(16).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(17).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(18).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(19).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(2).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(20).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(21).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(22).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(23).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(24).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(25).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(26).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(27).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(28).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(29).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(3).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(4).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(5).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(6).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(7).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(8).czi
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3D7_NHS405_ERD2_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(9).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(10).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(11).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(12).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(13).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(14).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(15).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(16).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(17).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(18).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(19).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(2).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(20).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(21).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(22).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(23).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(24).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(25).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(26).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(27).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(28).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(29).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(3).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(30).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(31).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(32).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(4).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(5).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(6).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(7).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(8).czi
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3D7_NHS405_HistoneH3_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(9).czi
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3D7_NHS405_MSP1_488_GAP45_555_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_MSP1_488_GAP45_555_SytoxRed_Airyscan_Processed_(2).czi
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3D7_NHS405_MSP1_488_GAP45_555_SytoxRed_Airyscan_Processed_(3).czi
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3D7_NHS405_MSP1_488_IMC1g_555_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_MSP1_488_IMC1g_555_SytoxRed_Airyscan_Processed_(2).czi
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3D7_NHS405_MSP1_488_IMC1g_555_SytoxRed_Airyscan_Processed_(3).czi
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3D7_NHS405_RAMA488_RON4_555_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_RAMA488_RON4_555_SytoxRed_Airyscan_Processed_(2).czi
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3D7_NHS405_RAMA488_RON4_555_SytoxRed_Airyscan_Processed_(3).czi
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3D7_NHS405_RAP1_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_RAP1_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(10).czi
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3D7_NHS405_RAP1_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(11).czi
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3D7_NHS405_RAP1_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(12).czi
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3D7_NHS405_RAP1_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(13).czi
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3D7_NHS405_RAP1_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(14).czi
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3D7_NHS405_RAP1_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(15).czi
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3D7_NHS405_RAP1_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(16).czi
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3D7_NHS405_RAP1_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(17).czi
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3D7_NHS405_RAP1_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(18).czi
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3D7_NHS405_RAP1_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(9).czi
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3D7_NHS405_RAP1_488_ERD2_555_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_RAP1_488_ERD2_555_SytoxRed_Airyscan_Processed_(3).czi
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3D7_NHS405_RAP1_488_ERD2_555_SytoxRed_Airyscan_Processed_(6).czi
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3D7_NHS405_RON4_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_RON4_488_RAMA_555_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_RON4_488_RAMA_555_SytoxRed_Airyscan_Processed_(3).czi
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3D7_NHS405_RON4_488_RAMA_555_SytoxRed_Airyscan_Processed_(4).czi
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3D7_NHS405_Tubulin488_BODIPYTRc_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_Tubulin488_BODIPYTRc_SytoxRed_Airyscan_Processed_(38).czi
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3D7_NHS405_Tubulin488_BODIPYTRc_SytoxRed_Airyscan_Processed_(9).czi
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3D7_NHS405_Tubulin488_HsCentrin1_555_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_Tubulin488_HsCentrin1_555_SytoxRed_Airyscan_Processed_(6).czi
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3D7_NHS405_Tubulin488_HsCentrin1_555_SytoxRed_Airyscan_Processed_(8).czi
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3D7_NHS405_Tubulin488_PolyE555_SytoxRed_Airyscan_Processed_(1).czi
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3D7_NHS405_Tubulin488_PolyE555_SytoxRed_Airyscan_Processed_(10).czi
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3D7_NHS405_Tubulin488_PolyE555_SytoxRed_Airyscan_Processed_(9).czi
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ACP-TS-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(1).czi
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ACP-TS-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(19).czi
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ACP-TS-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(46).czi
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ACP-TS-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(50).czi
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ACP-TS-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(54).czi
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ACP-TS-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(55).czi
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ACP-TS-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(56).czi
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ACP-TS-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(57).czi
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ACP-TS-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(58).czi
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ACP-TS-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(59).czi
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ACP-TS-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(60).czi
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ACP-TS-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(61).czi
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ACP-TS-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(62).czi
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ACP-TS-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(63).czi
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ACP-TS-GFP_NHS405_GFP488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(1).czi
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ACP-TS-GFP_NHS405_GFP488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(2).czi
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ACP-TS-GFP_NHS405_GFP488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(3).czi
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ATPd_smHA_NHS405_HA488_BIP568_SytoxRed_Airyscan_Processed_(1).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(17).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(18).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(20).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(21).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(22).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(23).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(24).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(25).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(26).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(27).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(28).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(29).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(3).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(30).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(31).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(32).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(33).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(34).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(35).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(36).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(37).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(38).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(39).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(4).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(40).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(41).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(42).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(43).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(44).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(45).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(46).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(47).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(48).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(49).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(5).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(50).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(51).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(52).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(53).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(54).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(55).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(56).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(57).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(58).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(59).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(6).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(60).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(61).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(62).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(63).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(64).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(65).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(66).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(67).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(68).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(69).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(7).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(70).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(71).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(72).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(73).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(74).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(75).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(76).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(77).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(78).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(79).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(80).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(81).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(83).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(91).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(92).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(93).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(94).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(95).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(96).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(97).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(98).czi
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ATPd_smHA_NHS405_HA488_BODIPYTRc_SytoxRed_Airyscan_Processed_(99).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(1).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(13).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(14).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(15).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(16).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(17).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(18).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(19).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(20).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(21).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(22).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(23).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(24).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(25).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(26).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(27).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(28).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(3).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(4).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(5).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(6).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(7).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(8).czi
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ATPd_smHA_NHS405_HA488_MitoTrackerCMTMRos_SytoxRed_Airyscan_Processed_(9).czi
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CINCHsmV5_NHS405_GAP45_488_BODIPYTRc_SytoxRed_Airyscan_Processed_(1).czi
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CINCHsmV5_NHS405_GAP45_488_V5_555_SytoxRed_Airyscan_Processed_(4).czi
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CINCHsmV5_NHS405_GAP45_488_V5_555_SytoxRed_Airyscan_Processed_(5).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(12).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(13).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(14).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(15).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(16).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(17).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(18).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(19).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(20).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(21).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(22).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(23).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(24).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(25).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(26).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(27).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(28).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(29).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(3).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(30).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(31).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(32).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(33).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(34).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(35).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(36).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(37).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(38).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(39).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(4).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(40).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(41).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(42).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(43).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(44).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(45).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(46).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(47).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(48).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(49).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(5).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(50).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(51).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(52).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(53).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(54).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(55).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(56).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(57).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(6).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(7).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(8).czi
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K13-GFP_NHS405_GFP488_BODIPYTRc_SytoxRed_Airyscan_Processed_(9).czi
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README.md
Abstract
Apicomplexan parasites exhibit tremendous diversity in much of their fundamental cell biology, but study of these organisms using light microscopy is often hindered by their small size. Ultrastructural expansion microscopy (U-ExM) is a microscopy preparation method that physically expands the sample ∼4.5x. Here, we apply U-ExM to the human malaria parasite Plasmodium falciparum during the asexual blood stage of its lifecycle to understand how this parasite is organized in three-dimensions. Using a combination of dye-conjugated reagents and immunostaining, we have catalogued 13 different P. falciparum structures or organelles across the intraerythrocytic development of this parasite and made multiple observations about fundamental parasite cell biology. We describe that the microtubule organizing center (MTOC) and its associated proteins anchor the nucleus to the parasite plasma membrane during mitosis. Furthermore, the rhoptries, Golgi, basal complex, and inner membrane complex, which form around this anchoring site while nuclei are still dividing, are concurrently segregated and maintain an association to the MTOC until the start of segmentation. We also show that the mitochondrion and apicoplast undergo sequential fission events while maintaining an MTOC association during cytokinesis. Collectively, this study represents the most detailed ultrastructural analysis of P. falciparum during its intraerythrocytic development to date, and sheds light on multiple poorly understood aspects of its organelle biogenesis and fundamental cell biology.
README: Atlas of Plasmodium falciparum intraerythrocytic development using expansion microscopy
https://doi.org/10.5061/dryad.9s4mw6mp4
This dataset contains all images that support the study entitled "Atlas of Plasmodium falciparum intraerythrocytic development using expansion microscopy" published in eLife (https://doi.org/10.7554/eLife.88088.1)
Description of the data and file structure
Each of the files included in this dataset is a .czi file corresponding to an Airyscan-processed image of a single Plasmodium falciparum parasite.
Files are named using the following convention:
Parasite line_ Fluorescence Channel 1_Fluorescence Channel 2_Fluorescence Channel 3_Fluorescence Channel 4_Airyscan Processed (Image number).czi
Fluorescence channels are ordered from shortest wavelength (in nm) to longest. Channels corresponding to antibody stains have the name of the target protein followed by the wavelength of the conjugated fluorophore. Image number does not follow any particular order, and only represents the number of images for that particular parasite line and fluorophore combination.
For example:
3D7_NHS405_Aldolase488_BODIPYTRc_SytoxRed_Airyscan Processed (1).czi
A small number of images in the dataset were of parasites fixed using a combination of paraformaldehyde (PFA) and glutaraldehyde (glut). These use the naming convention:
Parasite line (PFA-Glut)_Fluorescence Channel 1_Fluorescence Channel 2_Fluorescence Channel 3_Fluorescence Channel 4_Airyscann Processed (Image number).czi
Images of the following parasite lines are represented in this database:
Plasmodium falciparum 3D7
Plasmodium falciparum 3D7_ACP-TS-GFP (referred to as ACP-TS-GFP in the dataset)
Plasmodium falciparum 3D7_ATP_Synthase_F0 Subunit_d(Pf3D7_0311800)_smHA (referred to as ATPd in the dataset)
Plasmodium falciparum 3D7_CINCH(Pf3D7_0407800)_smV5 (referred to as CINCHsmV5 in the dataset)
Plasmodium falciparum 3D7_Kelch13WT_GFP (referred to as K13-GFP in the dataset)
Commonly used colours for fluorescence channels represented in this dataset:
NHS Ester Alexa Fluor 405 (Protein stain) = Inverse Greyscale
BODIPY Tr Ceramide (Lipid stain) = White
Protein of interest A = Magenta
SYTOX Deep Red (DNA stain) = Cyan
Protein of interest B or MitoTracker CMTMRos = Yellow
The following abbreviations are used in the naming of images of the dataset:
NHS = N-hydroxysuccinimdyl ester Alexa Fluor 405
BODIPY TRc = BODIPY Texas Red Ceramide
Sytox Red = SYTOX Deep Red
EBA175 = Erythrocyte Binding Antigen 175
AMA1 = Apical Membrane Antigen 1
RAMA = Rhoptry Apical Membrane Antigen
RAP1 = Rhoptry Associated Protein 1
IMC1g = Inner-membrane Complex Protein 1g
MSP1 = Merozoite Surface Protein 1
GAP45 = Glideosome Associated Protein 45
RON4 = Rhoptry Neck Protein 4
GFP = Green Fluorescence Protein
ACP-TS = Acyl carrier protein apicoplast targeting signal
ATPd = ATP Synthase Subunit F0 d
sm = spaghetti monster tag
K13 = Kelch13
PFA = Paraformaldehyde
Glut = Glutaraldehyde
Sharing/Access information
All data in this dataset was generated for, and underlies the conclusion of the following study:
Methods
This dataset comprises 647 images of Plasmodium falciparum parasites acquired using Airyscan microscopy on either a Zeiss LSM 800, or Zeiss LSM 900 microscope using a 63x Plan Apochromat 1.4NA objective lens. All images in this dataset have been Airyscan processed in 3D using the "standard" filter setting. Raw, non-airyscanned, images are available upon request, but have not been included in this dataset as they cannot be used in many of the most commonly used microscopy image analysis programs. Channels for fluorescent markers have been changed to reflect the colouring system used in the corresponding manuscript. While image colour combinations vary for some images, they broadly follow the trend: Cyan = SYTOX Deep Red (DNA stain), Magenta = antibody stain of interest (typically Alexa Fluor 488), Inverse greyscale = NHS ester Alexa Fluor 405 (protein stain), White = BODIPYTexasRed Ceramide (lipid stain), Yellow = second antibody stain of interest (typically Alexa Fluor 555), or MitoTracker CMTMRos (mitochondrial stain).