Data from: "Transcriptome sequencing of the Queensland fruit fly, Bactrocera tryoni (Diptera: Tephritidae)" in Genomic Resources Notes accepted 1 December 2013 to 31 January 2014

Nagalingam, Kumaran, University of Innsbruck

Prentis, Peter, Queensland University of Technology

Clarke, A. R., University of Innsbruck

Publication date: March 28, 2014

Publisher: Dryad

https://doi.org/10.5061/dryad.b21r5

Citation

Nagalingam, Kumaran; Prentis, Peter; Clarke, A. R. (2014), Data from: "Transcriptome sequencing of the Queensland fruit fly, Bactrocera tryoni (Diptera: Tephritidae)" in Genomic Resources Notes accepted 1 December 2013 to 31 January 2014, Dryad, Dataset, https://doi.org/10.5061/dryad.b21r5

Abstract

[No abstract filled]

Usage Notes

Contigs from Library 1

An unannotated version of the trascriptome assembly (contigs and unigenes) is presented here.

F-Contig.fa

Contigs from Library 2

An unannotated version of the trascriptome assembly (contigs and unigenes) is presented here.

UF-Contig.fa

All-Unigene

An unannotated version of the trascriptome assembly (contigs and unigenes) is presented here.

Sequence assembly and Contig annotation

The raw reads were used to generate a de novo assembly. High quality reads were then assembled into contigs and tese contigs were then further assembled into unigenes using paired end information to combine contigs that arose from the same transcript.

annotation.xls

Blast hits

All unigenes were screened against protein databases Nr, SwissProt, KEGG and COG using BLASTx (e-value<10-5) and against nucleotide database Nt using BLASTn (e-value<10-5). The best hits were used to determine the sequence direction of the unigenes.

All-Unigene.blast.cds.fa

Location

Brisbane

Department of Agriculture, Fisheries and Forestry

Australia