MTT raw data for respective ganoderma chromatography fractions
Data files
Apr 30, 2024 version files 10.56 KB
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PLOS_data.csv
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README.md
Abstract
Ganoderma is a genus of medically important fungus that contains at least 80 species, many of which have not been properly evaluated for their anticancer potential. This study was conducted to assess the cytotoxic activity of the mycelial biomass of Ganoderma weberianum-sichuanese isolated from the Lower Volta River Basin of Ghana. The nuclear ribosomal internal transcribed spacer (ITS) region was analyzed to determine the phylogenetic position of this native ganoderma isolate. We then tested its cytotoxic activity against the human carcinoma cell line PC-3 (human prostate), Jurkat (human T lymphoblastoid cell line), derived from an acute T cell leukemia, and PMDC05, a plasmacytoid dendritic cell (pDC) derived from acute leukemia using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The ITS phylogenetic analysis demonstrated that this native Ghanaian ganoderma isolate belongs to the Ganoderma weberianum-sichuanese species complex. Fractions of the mycelial biomass were found to inhibit significantly (≤ 0.05%) the proliferation and survival of the three cancer cell lines, PC-3, PMDC05, and Jurkat with increasing concentrations and with IC50 values of 27.73 ± 5.25, 21.31 ± 2.40 and 17.09 ± 0.86 μg/mL, respectively compared to Chang liver cells (CVCL_0238) with an IC50 value of 75.41 ± 1.95 μg/mL. To the best of our knowledge, these findings demonstrated for the first time, that specific constituents of Ganoderma weberianum-sichuanese are selectively cytotoxic to the three human cancer cell lines, suggesting their potential efficacy in the treatment of malignancies. Future studies to isolate and characterize the biologically active molecules are warranted.
README: MTT raw data for respective ganoderma chromatography fractions
https://doi.org/10.5061/dryad.b5mkkwhmq
The data presented here is the raw data for the MTT assay in an excel format. Each cell line and the respective concentrations of the respective ganoderma chromatography fractions and their concentrations are listed in the respective columns.
Description of the data and file structure
Mean and standard deviation are given for each respective fraction.
Description
MTT Data of Extract and Fractions of Ganoderma weberianum-sichuanese
· S/N: Sample number
· Concentration (μg/ml): Concentration of dried extract and fractions in micrograms per milliliter
· Cell Viability Percentage (%): Cell growth or survival effect of extract and fractions measured by MTT assay
· Chang: Chang liver cells (CVCL_0238)
· Jurkat: human T lymphoblastoid cell line, derived from e derived from an acute T cell leukemia
· PMDCO5: a plasmacytoid dendritic cell (pDC) derived from acute leukemia
· PC-3: human carcinoma cell line PC-3 (human prostate)
· Mean: Mean values of MTT assay performed in triplicate on Jurkat, PC-3, PMDCO5, and Chang Liver cells.
· STDEV: standard deviations of MTT assay performed in triplicate on Jurkat, PC-3, PMDCO5, and Chang Liver cells.
· GL-CO1: Crude ethanol extract of Ganoderma weberianum-sichuanese
· GL-C1to C7: Seven fractions from crude ethanol extract of Ganoderma weberianum-sichuanese obtained by silica gel column chromatography using differing mixtures of solvents of increasing polarity
Methods
Cell viability upon treatment with respective chromatography fractions of Ganoderma weberianum-sichuanese extracts was established using the MTT assay.