Estimation of lifelong metabolic rates in marine fish: A combination of oxygen consumption measurements and δ13C metabolic proxy derived from vertebral structural carbonates
Data files
Jan 03, 2025 version files 26.25 KB
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black_porgy_oxygen_consumption_measurement.csv
2.70 KB
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black_porgy_stable_isotope_analysis.csv
13.50 KB
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README.md
10.05 KB
Jan 03, 2025 version files 26.24 KB
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black_porgy_oxygen_consumption_measurement.csv
2.70 KB
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black_porgy_stable_isotope_analysis.csv
13.50 KB
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README.md
10.05 KB
Abstract
Adjustments in the metabolism of marine fish are associated with the complexity of resource availability, prey-predator relationships, and biotic and abiotic interactions in the natural environment. To investigate the relationship between metabolism and body mass, this study used a conventional method to estimate the oxygen consumption rate (reflecting the resting metabolic rate) in black porgy, Acanthopagrus schlegelii, over a year of rearing. In addition, we developed a novel metabolic proxy using the δ13C values of vertebral structural carbonates to monitor lifelong metabolic changes. The oxygen consumption measurements followed a decreasing mass-dependent trend and yielded a mass-specific allometric exponent scaling (−0.24). By integrating the oxygen consumption with the advanced δ13C metabolic proxy, we established a decay model in an increasing form to describe the relationship of two measurements, and it could be further used in wild fishes and broaden the metabolic studies in marine vertebrates.
README: Estimation of lifelong metabolic rates in marine fish: A combination of oxygen consumption measurements and δ13C metabolic proxy derived from vertebral structural carbonates
https://doi.org/10.5061/dryad.bnzs7h4jx
The laboratory-controlled experiment was conducted on black porgy, Acanthopagrus schlegelii for over a year. Fish were measured for oxygen consumption rate and stable carbon isotope metabolic proxy, and two measurements (oxygen consumption rate and stable isotope analysis) are provided separately as two CSV files.
Description of the data and file structure
black porgy_oxygen consumption measurement:
The dataset includes two groups of black porgy in the experiment. The first group of 100 fish had 6–8 individuals randomly selected in the first three months to measure their resting metabolic rate (RMR) and determine the appropriate size for tagging in subsequent experiments. In the second group, 20 fish were microchipped (AVID, USA) at the age of 9 months on the basis of their size suitability for tagging determined from the first group’s data. Every oxygen consumption measurement was accompanied by the record of the fish's standard length, total length, body weight and water temperature.
| Column name | Description | Units | Data format | Missing data code |
|---|---|---|---|---|
| ID | Fish individual ID number | - | Category | - |
| Group | The two experimental groups in the rearing. Group 1: 6-8 individuals were randomly selected in the first three months for oxygen consumption measurements. Group 2: eight fish with tags. | - | Category | - |
| Weight | Fish weight when the individual was measured the oxygen consumption. | g | Number | - |
| Standard length | Fish standard length when the individual was measured the oxygen consumption. | cm | Number | - |
| Total length | Fish total length when the individual was measured the oxygen consumption. | cm | Number | - |
| Temperature | Temperature records when the fish was measured the oxygen consumption rate | °C | Number | - |
| RMR | Resting metabolic rate. The oxygen consumption rate was measured while the fish was in a resting state. | mg O2/h/kg | Number | NA, the measurement of RMR was not successful during the experiment. |
| Log(temperature corrected RMR) | The resting metabolic rate was temperature-corrected following the core equation of the metabolic theory of ecology. The temperature-corrected RMR was expressed as the logarithm, specifically using base 10. | mg O2/h/kg | Number | NA, the measurement of RMR was not successful during the experiment. |
black porgy_stable isotope analysis:
The fish samples were derived from the second group in the previous dataset The bone was sectioned for the signals at different life stages. First, the, section length of bone was used to reconstruct the fish length and body weight. Also, fish length was used to deduce the water temperature during the rearing because the fish was tagged. Each bone section was analysed with a stable carbon isotope value (d13C). The water DIC and food were also analysed with a stable carbon isotope value (DIC d13C and food d13C, respectively) with standard deviations of pooled samples. With the known 13C values of bone, DIC and food, the metabolic proxy (Cresp) was calculated.
| Column name | Description | Units | Data format | Missing data code |
|---|---|---|---|---|
| ID | Fish individual ID number | - | Category | - |
| Section number | The order of sectioned vertebral centrum. | - | Number | - |
| Section length | The percentage of section length compared to the total length of vertebral centrum. | % | Number | - |
| Reconstructed length | The fish total length was reconstructed by the vertebral section length. | cm | Number | - |
| Reconstructed weight | The fish weight was reconstructed by fish length. | kg | Number | - |
| carbonate wt | The weight percentage of structural carbonate in each vertebral section. | % | Number | - |
| d13C | Stable carbon isotope value of vertebral sections. | ‰ | Number | - |
| d18O | Stable oxygen isotope value of vertebral sections. | ‰ | Number | NA, the isotope values are unreliable because of low signal intensity. |
| d13C stdev | The standard deviation of stable carbon isotope values. | ‰ | Number | - |
| Cresp | The metabolic proxy was obtained by d13C values. | - | Number | - |
| Cresp stdev | The standard deviation of metabolic proxy. | - | Number | - |
| Reconstructed MO2_mean | The mean of oxygen consumption rate was reconstructed by the Cresp values. | mg O2/h/kg | Number | - |
| Reconstructed MO2_sd | The standard deviation of reconstructed oxygen consumption rate. | mg O2/h/kg | Number | - |
| DIC d13C | The mean stable carbon isotope value of water DIC | ‰ | Number | - |
| DIC d13C stdev | The standard deviation of stable carbon isotope value of water DIC | ‰ | Number | - |
| Food d13C | The mean stable carbon isotope value of food | ‰ | Number | - |
| Food d13C stdev | The standard deviation of stable carbon isotope value of food | ‰ | Number | - |
| Temperature | The mean temperature of rearing water | °C | Number | - |
| Temperature stdev | The standard deviation of the temperature of rearing water | °C | Number | - |
Methods
The study investigated the ontogenetic metabolism of the marine species black porgy (Acanthopagrus schlegelii) by measuring the oxygen consumption rate and using a novel δ13C metabolic proxy. Specifically, this study (1) monitored the metabolic level during ontogeny in a controlled environment, (2) estimated the allometric exponent of black porgy to understand how metabolic changes are linked with fish growth, and (3) evaluated the δ13C value recorded in structural carbonate as a potential metabolic proxy.
The oxygen measurement was conducted using a fibre optic oxygen meter (OXY-4; PreSens, Germany). The isotope analyses were performed using Finnigan GasBench II equipped with Delta V Plus IRMS and EA-IRMS (Thermo Fisher Scientific, Bremen, Germany).