Data from: Gating-spring stiffness increases outer-hair-cell bundle stiffness, damping, and receptor current
Data files
Dec 14, 2024 version files 1.65 GB
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3DOHB_012624.CSV
125.38 MB
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3DOHB_012624.mx
1.09 GB
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mut3DOHB_062724.CSV
143.18 MB
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mut3DOHB_062724.mx
158.90 MB
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mutIdenticalColumnsOHB_071224.CSV
123.39 MB
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mutIdenticalColumnsOHB_071224.mx
2.09 MB
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README.md
1.95 KB
Abstract
In our ears, outer-hair-cell bundles (OHBs) convert sound-induced forces into receptor currents that drive cochlear amplification, the process responsible for the micropascal-scale threshold and million-fold dynamic range of hearing. OHBs rely on gating springs to open mechanoelectrical-transduction (MET) ion channels, through which the receptor current flows. OHBs have larger gating-spring stiffnesses than other types of hair bundles, but we have a poor understanding of how gating-spring stiffness contributes to OHB mechanics and receptor-current regulation. Using experimentally-constrained mathematical models of the OHB, we show that the increased gating-spring stiffness in an OHB increases its stiffness and damping. The OHB's 3D morphology reduces the contribution of gating-spring stiffness to OHB stiffness, reduces the contribution of MET-channel gating to OHB stiffness and damping, but causes additional OHB damping that rises with gating-spring stiffness. Gating-spring stiffness increases the OHB's receptor current but decreases its displacement-current dynamic range. Strikingly, the OHB's 3D morphology causes its force-current dynamic range to decrease with gating-spring stiffness. Our results suggest a trade-off between threshold and dynamic range regulated by OHB gating-spring stiffness. The mathematical modeling code supporting the results is provided here. Three types of mathematical models are included, a 3D wild-type OHB model, 3D OHB models with different gating-spring stiffnesses, and identical-columns OHB models with different gating-spring stiffnesses. The code generated three data files.
README: Gating-Spring Stiffness Increases Outer-Hair-Cell Bundle Stiffness, Damping, and Receptor Current
https://doi.org/10.5061/dryad.bnzs7h4kz
Description of the data and file structure
Simulation data was created using mathematical models. The data is available in the mx files and is in Wolfram Language serialized package format. This data can be read-in by the corresponding code files nb, which can be opened using Mathematical 13.3 or later. The data in the mx files is also available in csv files, which can be opened as a text files. The nb files can also be opened using the freely available Wolfram Player.
Files and variables
Three types of mathematical models of outer-hair-cell bundles are included.
3DOHB.nb contains the code for the wild-type outer-hair-cell bundle 3D model.
3DOHB_012624.mx contains the data for the wild-type outer-hair-cell bundle 3D model.
3DOHB_012624.CSV contains the data for the wild-type outer-hair-cell bundle 3D model.
mut3DOHB.nb contains the code for 3D outer-hair-cell bundle models with different gating-spring stiffnesses.
mut3DOHB_062724.mx contains the data for 3D outer-hair-cell bundle models with different gating-spring stiffnesses.
mut3DOHB_062724.CSV contains the data for 3D outer-hair-cell bundle models with different gating-spring stiffnesses.
mutIdenticalColumnsOHB.nb contains the code for identical-columns outer-hair-cell bundle models with different gating-spring stiffnesses.
mutIdenticalColumnsOHB_071224.mx contains the data for identical-columns outer-hair-cell bundle models with different gating-spring stiffnesses.
mutIdenticalColumnsOHB_071224.CSV contains the data for identical-columns outer-hair-cell bundle models with different gating-spring stiffnesses.
Code/software
Mathematica 13.3 or later, or the free Wolfram Player will open the nb files. The data files are incorporated at the top of the nb code files.
Methods
Mathematical modeling code was written in Mathematica 13.3 and the data from the code was saved in mx files. The data from the mx files was exported to CSV files, which can be read using free software.