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Assessment of serum hormone levels in female patients with acne vulgaris


Nguyen Phu, Cuong et al. (2022), Assessment of serum hormone levels in female patients with acne vulgaris, Dryad, Dataset,


Introduction: Acne is a chronic inflammatory disorder of the pilosebaceous unit with differential pathogenesis. And hormone plays a part role in the pathogenesis of acne. The objective of this study was valuation of the serum testosterone, estradiol, progesterone levels in women with acne vulgaris. 

Methods: We conducted a cross-sectional study and 175 women with acne vulgaris were examined, their serum estradiol, progesterone, testosterone were analyzed by chemiluminescence technique and compared with the healthy control group. 

Results: Increased serum hormone levels in women with acne vulgaris were accounted for 29.7%, and hyperandrogenism was accounted for 16.0% of cases. We found significant differences in testosterone levels (mean value, 55.67±25.56 versus 38.37±10.16 ng/dL, p<0.05) respectively in the acne group and the control group. However, the estradiol level of the acne group (323.15±93.31 pmol/L) was lower than the control group (370±58.88 pmol/L) with p<0.05). No statistically significant differences were found for progesterone (0.60±0.38 versus 0.50±0.15 ng/mL, p>0.05) levels. Moreover, we did not find the relationship between serum hormone levels and the severity of acne vulgaris

Conclusion: This study showed that the female acne vulgaris patients may have high serum testosterone levels and low serum estradiol levels compared with those of female controls. However, hormone alterations had no correlation with the acne grades.


The data were collected randomly from acne patients visiting the clinical room of the Dermatology department of 103 military hospital, Vietnam, in 2019. 210 female participants were enrolled and divided into 175 female patients with acne vulgaris and 35 healthy controls.

The acne grading score according to Karen McKoy (2008) are as follows: Mild: less than 20 noninflammatory lesions or less than 15 inflammatory lesions or less than 30 total kinds of lesions; Moderate: 20–100 noninflammatory lesions or 15–50 inflammatory lesions or 30–125 total kinds of lesions. Severe: more than 05 papules/cysts or more than 100 noninflammatory lesions/more than 50 inflammatory lesions or more than 125 total kinds of lesions.

Assessment technique of plasma hormone levels: 4 mL of venous blood were collected at 08:00h and during the follicular phase of the menstrual cycle (2nd to 4th day of menses)Blood samples are contained in a tube with EDTA anticoagulant solution. After being incubated at room temperature for 10–20 minutes, samples are centrifuged for about 20 minutes at 2000–3000 rpm. After centrifuging, taking the plasma part for testing, we used direct chemiluminescent technology for measuring hormone levels in the Unicel® DXI800 machine. Units: testosterone for ng/dL, estradiol for pmol/L, progesterone for ng/mL.

Usage Notes

The readme file contains an explanation of each of the variables in the dataset, its measurement units, and -if it concerns a derived variable (displayed in grey) - the way it was calculated from the primary data (displayed in black). #NA =  values not available. Information on how the measurements were done can be found in the associated manuscript referenced above.