Data from: Climate oscillations, glacial refugia, and dispersal ability: factors influencing the genetic structure of the least salmonfly, Pteronarcella badia (Plecoptera), in Western North America
Sproul, John S., Oregon State University
Houston, Derek D., Department of Ecology, Evolution and Organismal Biology, Ames, USA
Nelson, C. Riley, Brigham Young University
Evans, R. Paul, Brigham Young University
Crandall, Keith A., University of Washington
Shiozawa, Dennis K., Brigham Young University
Published Dec 09, 2015 on Dryad.
Cite this dataset
Sproul, John S. et al. (2015). Data from: Climate oscillations, glacial refugia, and dispersal ability: factors influencing the genetic structure of the least salmonfly, Pteronarcella badia (Plecoptera), in Western North America [Dataset]. Dryad. https://doi.org/10.5061/dryad.c1sd4
Background: Phylogeographic studies of aquatic insects provide valuable insights into mechanisms that shape the genetic structure of communities, yet studies that include broad geographic areas are uncommon for this group. We conducted a broad scale phylogeographic analysis of the least salmonfly Pteronarcella badia (Plecoptera) across western North America. We tested hypotheses related to mode of dispersal and the influence of historic climate oscillations on population genetic structure. In order to generate a larger mitochondrial data set, we used 454 sequencing to reconstruct the complete mitochondrial genome in the early stages of the project. Results: Our analysis revealed high levels of population structure with several deeply divergent clades present across the sample area. Evidence from five mitochondrial genes and one nuclear locus identified a potentially cryptic lineage in the Pacific Northwest. Gene flow estimates and geographic clade distributions suggest that overland flight during the winged adult stage is an important dispersal mechanism for this taxon. We found evidence of multiple glacial refugia across the species distribution and signs of secondary contact within and among major clades. Conclusions: This study provides a basis for future studies of aquatic insect phylogeography at the inter-basin scale in western North America. Our findings add to an understanding of the role of historical climate isolations in shaping assemblages of aquatic insects in this region. We identified several geographic areas that may have historical importance for other aquatic organisms with similar distributions and dispersal strategies as P. badia. This work adds to the ever-growing list of studies that highlight the potential of next-generation DNA sequencing in a phylogenetic context to improve molecular data sets from understudied groups.
A NEXUS file written for Mesquite with a concatenated alignment and for all haplotypes which was used to reconstruct phylogenetic relationships (in RAxML) of populations of Pteronarcella badia and its sister species across western North America.
The input file for BEAST containing all haplotypes which was used to infer divergence among populations of Pteronarcella badia across western North America.
A BEAST input file used to infer recent demographic history via Bayesian Skyline analysis of Pteronarcella badia populations in western North America.
Three input files used to infer migration rates of Pteronarcella badia across, and within several major drainage basin boundaries in Western North America.
The input file used to generate a haplotype network of Pteronarcella badia in TCS using a concatenated dataset of mitochondrial genes.
The input file used to generate a haplotype network of Pteronarcella badia in TCS using a portion of 28S rRNA.
Input files for IBD (Isolation-by-Distance) web server (http://ibdws.sdsu.edu/~ibdws/distances.html) used to test for isolation-by-distance as a determinant of population structure within each major clade of Pteronarcella badia sequences.
Sample Locality Data
A table that includes sample locality for all specimens in the study.