Data from: Exposure to opposing temperature extremes causes comparable effects on Cardinium density but contrasting effects on Cardinium-induced cytoplasmic incompatibility
Doremus, Matthew R.
Kelly, Suzanne E.
Hunter, Martha S.
Published Aug 29, 2019 on Dryad.
Cite this dataset
Doremus, Matthew R.; Kelly, Suzanne E.; Hunter, Martha S. (2019). Data from: Exposure to opposing temperature extremes causes comparable effects on Cardinium density but contrasting effects on Cardinium-induced cytoplasmic incompatibility [Dataset]. Dryad. https://doi.org/10.5061/dryad.c1v64np
Terrestrial arthropods, including insects, commonly harbor maternally inherited intracellular symbionts that confer benefits to the host or manipulate host reproduction to favor infected female progeny. These symbionts may be especially vulnerable to thermal stress, potentially leading to destabilization of the symbiosis and imposing costs to the host. For example, increased temperatures can reduce the density of a common reproductive manipulator, Wolbachia, and the strength of its crossing incompatibility (cytoplasmic incompatibility, or CI) phenotype. Another manipulative symbiont, Cardinium hertigii, infects ~ 6-10% of Arthropods, and also can induce CI, but there is little homology between the molecular mechanisms of CI induced by Cardinium and Wolbachia. Here we investigated whether temperature disrupts the CI phenotype of Cardinium in a parasitic wasp host, Encarsia suzannae. We examined the effects of both warm (32°C day/ 29°C night) and cool (20°C day/ 17°C night) temperatures on Cardinium CI and found that both types of temperature stress modified aspects of this symbiosis. Warm temperatures reduced symbiont density, pupal developmental time, vertical transmission rate, and the strength of both CI modification and rescue. Cool temperatures also reduced symbiont density, however this resulted in stronger CI, likely due to cool temperatures prolonging the host pupal stage. The opposing effects of cool and warm-mediated reductions in symbiont density on the resulting CI phenotype indicates that CI strength may be independent of density in this system. Temperature stress also modified the CI phenotype only if it occurred during the pupal stage, highlighting the likely importance of this stage for CI induction in this symbiosis.
This file contains data from crossing experiments using wasps (Encarsia suzannae, formerly E. pergandiella) infected with a heritable bacterium, Cardinium hertigii. These experiments tested the effect of exposure to different temperature regimens on a crossing incompatibility caused by C. hertigii between infected males and uninfected females, known as CI. CI is rescued when males mate with infected females (control or rescue cross in data file). Both male and female wasps were exposed to temperature stress in separate experiments, and a third experiment, using temperature "shocks" was also conducted using male hosts. The duration of pupal development and Cardinium density within the host are also included.