Data from: Compromised femoral and lumbovertebral bone in the Dp(16)1Yey Down syndrome mouse model
Data files
Sep 03, 2024 version files 14.69 KB
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Dp(16)1Yey_Data_for_DRYAD_Upload.csv
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README.md
Abstract
Down syndrome (DS), affecting ~1 in 800 live births, is caused by the triplication of human chromosome 21 (Hsa21). Individuals with DS have skeletal features including craniofacial abnormalities and decreased bone mineral density (BMD). Lowered BMD can lead to increased fracture risk, with common fracture points at the femoral neck and lumbar spine. While the femur has been studied in DS mouse models, there is little research done on the vertebrae despite evidence that humans with DS have affected vertebrae. Additionally, it is important to establish when skeletal deficits occur to find times of potential intervention. The Dp(16)1Yey DS mouse model has all genes triplicated on mouse chromosome 16 orthologous to Hsa21 and displayed deficits in long bone, including trabecular and cortical deficits in male but not female mice, at 12 weeks. We hypothesized that the long bone and lumbovertebral microarchitecture would exhibit deficits in Dp(16)1Yey mice compared to control mice, long bone strength would be diminished in Dp(16)1Yey mice, and there would be a sexual dimorphism in these parameters at 6 weeks. The trabecular region of the 4th lumbar (L4) vertebra and the trabecular and cortical regions of the femur were analyzed via micro-computed tomography and 3-point bending in 6-week-old male and female Dp(16)1Yey and littermate control mice. Trabecular and cortical deficits were observed in femurs from male Dp(16)1Yey mice, and cortical deficits were seen in femurs of male and female Dp(16)1Yey mice. Additionally, the L4 of male and female Dp(16)1Yey mice show trabecular deficits, which have not been previously reported in a DS mouse model. Our results indicate that skeletal deficits associated with DS occur early in skeletal development, are dependent on skeletal compartment and site, are sex dependent, and potential interventions should likely begin early in skeletal development of DS mouse models.
README: Data from: Compromised femoral and lumbovertebral bone in the Dp(16)1Yey Down syndrome mouse model
The following data was utilized in the following manuscript: (citation information)
Femoral length and mechanical parameters could not be completed for Yey58 and Yey66 due to being broken after uCT scanning. Trabecular parameters (BMD, BV/TV, Tb.Th, Tb.Sp, Tb.N) were reported for right femurs (Fem.)and 4th lumbar vertebra (Vert.) Cortical parameters (Tt.Ar, Ma.Ar, Ct.Ar, Ct.Ar/Tt.Ar, Ct.Th, Ps.Pm, Ec.Pm, Ct.TMD) were reported for right femurs. Mechanical parameters (remaining) were reported based on 3-point bend for right femurs. Three femurs (Yey55,Yey68,Yey77) did not reach a failure point (break), so failure points may be overestimated (they are reported for creation of the Force-Displacement and Stress-Strain curves).
Columns are as follows:
Sample ID
Age of animal at dissection in weeks
Sex where M = male and F = female
Genotype
Body weight (Bod.Weight) in grams
Femoral length (Fem.Length) in millimeters
Femoral bone mineral density (Fem.BMD) in g/cm^3
Femoral bone volume fraction (Fem.BV/TV) in %
Femoral trabecular thickness (Fem.Tb.Th) in mm
Femoral trabecular separation (Fem.Tb.Sp) in mm
Femoral trabecular number (Fem.Tb.N) in 1/mm
Total cortical cross-sectional area (Tt.Ar) in mm^2
Marrow area (Ma.Ar) in mm^2
Cortical bone area (Ct.Ar) in mm^2
Cortical area fraction (Ct.Ar/Tt.Ar) in %
Cortical bone thickness (Ct.Th) in mm
Periosteal bone perimeter (Ps.Pm) in mm
Endocortical bone perimeter (Ec.Pm) in mm
Cortical tissue mineral density (Ct.TMD) in hydroxyaptite(HA)g/cm^3
Vertebral bone mineral density (Vert.BMD) in g/cm^3
Vertebral bone volume fraction (Vert.BV/TV) in %
Vertebral trabecular thickness (Vert.Tb.Th) in mm
Vertebral trabecular separation (Vert.Tb.Sp) in mm
Vertebral trabecular number (Vert.Tb.N) in 1/mm
Yield force (force at yield point) in N
Ultimate force (force at ultimate point) in N
Failure force (force at failure point) in N
Displacement to yield [point] in um (micrometers)
Ultimate displacement (displacement to ultimate point) in um
Total displacement (displacement to failure point) in um
Stiffness (slope of linear section of Force-Displacement curve [prior to yield point]) in N/mm
Work to yield (area under the Force-Displacement curve prior to yield point) in mJ
Yield stress (stress to yield point) in MPa
Ultimate stress (stress to ultimate point) in MPa
Failure stress (stress to failure point) in MPa
Strain to yield [point] in ue (microstrain)
Ultimate strain (strain to ultimate point) in ue
Total strain (strain to failure point) in ue
Modulus (slope of linear section of Stress-Strain curve [prior to yield point]) in GPa
Resilience (area under the Stress-Strain curve prior to yield point) in MPa