Data from: Growth of maize on mixed nitrate and ammonium promotes auxin synthesis and biomass accumulation

Wang, Peng, China Agricultural University

Wang, Zhangkui, China Agricultural University

Pan, Qingchun, Huazhong Agricultural University

Sun, Xichao, China Agricultural University

Chen, Huan, China Agricultural University

Chen, Fanjun, China Agricultural University

Yuan, Lixing, China Agricultural University

Mi, Guohua, China Agricultural University

Published Feb 19, 2019 on Dryad. https://doi.org/10.5061/dryad.cd57c84

Cite this dataset

Wang, Peng et al. (2019). Data from: Growth of maize on mixed nitrate and ammonium promotes auxin synthesis and biomass accumulation [Dataset]. Dryad. https://doi.org/10.5061/dryad.cd57c84

Abstract

Mixed nitrate (NO3-) and ammonium (NH4+) as nitrogen source can improve plant growth. The underlying mechanism was explored by using metabolomics and transcriptome. Maize plants were grown hydroponically with three nitrogen forms (nitrate, 75/25 nitrate/ammonium and ammonium). Mixed N increased photosynthetic rate compared to nitrate supply, and had the highest leaf area, shoot and root biomass among the three nitrogen treatments. In shoot and root, the concentration of nitrogenous compounds (NH4+, Gln, Asn) and carbohydrates (sucrose, glucose, fructose) in plant with mixed N supply was higher than that with nitrate supply, but lower than that with ammonium supply. The activity of the related enzymes (GS, ASNS, PEPCase, invertase, AGPase) changed accordingly. Specifically, mixed N enhanced IAA synthesis via shikimic acid pathway, as indicated by the higher PEP and tryptophan compared to the other two treatments. The expression of corresponding genes involving IAA synthesis and response were up-regulated. Sole ammonium supply resulted in high level of Gln and Asn, starch, and T6P. It is concluded that, in addition to increase photosynthesis, mixed N supply enhances leaf growth via increasing IAA synthesis to build a big sink for C and N utilization, which, in turn, facilitates further C assimilation and N uptake.

Usage notes

Metabolites in the shoot and root identified by LC-MS under sole nitrate, 75/25 nitrate and ammonium ratio supply, and sole ammonium supply in 12d for maize (Zhengdan 958) seedlings in hydroponic condition.

Metabolites in the shoot and root identified by LC-MS under sole nitrate, 75/25 nitrate and ammonium ratio supply, and sole ammonium supply in 12d for maize (Zhengdan 958) seedlings in hydroponic condition. Using the R (V3.3.2) XCMS package to identify peaks identification, peaks filtration, peaks alignment. In among, the main parameters are bw=5, ppm=15, peakwidth=c(10,120), mzwid=0.015, mzdiff=0.01, method=”centWave”. Obtain a data matrix including information such as“mass to charge ration, m/z”, retention time and intensity. In among, positive ion mode obtains 16068 precursor molecules and negative ion mode obtains 5259 precursor molecules for each N forms. The data was exported to excel for subsequent analysis. SCK, ST1 and ST2 indicate the metabolites in the shoot of sole nitrate supply, mixed N supply and sole ammonium supply in 12d, respectively. RCK, RT1 and RT2 indicate the metabolites in the root of sole nitrate supply, mixed N supply and sole ammonium supply in 12d, respectively.

raw-negative-positive.csv

Supplementary Material

Supplementary tables and figures

Supplementarymaterial.pdf