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Data from: Phylogenomics using formalin-fixed and 100+ year old intractable natural history specimens

Citation

Ruane, Sara; Austin, Christopher C. (2017), Data from: Phylogenomics using formalin-fixed and 100+ year old intractable natural history specimens, Dryad, Dataset, https://doi.org/10.5061/dryad.cm28r

Abstract

Museum specimens provide a wealth of information to biologists, but obtaining genetic data from formalin-fixed and fluid-preserved specimens remains challenging. While DNA sequences have been recovered from such specimens, most approaches are time-consuming and produce low data quality and quantity. Here we use a modified DNA extraction protocol combined with high-throughput sequencing to recover DNA from formalin-fixed and fluid-preserved snakes that were collected over a century ago and for which little or no modern genetic materials exist in public collections. We successfully extracted DNA and sequenced ultraconserved elements (x̄ = 2,318 loci) from 10 fluid-preserved snakes and included them in a phylogeny with modern samples. This phylogeny demonstrates the general use of such specimens in phylogenomic studies and provides evidence for the placement of enigmatic snakes, such as the rare and never-before sequenced Indian Xylophis stenorhynchus. Our study emphasizes the relevance of museum collections in modern research and simultaneously provides a protocol that may prove useful for specimens that have been previously intractable for DNA sequencing.

Usage Notes

Funding

National Science Foundation, Award: DEB-1146033

Location

United States
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