Data from: Reevaluating monotypic Eleutherostylis from New Guinea and the Moluccas, and its inclusion in Grewia (Malvaceae: Grewioideae)
Data files
Dec 20, 2023 version files 102.78 KB
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README.md
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Wurdack_Dorr_EleutherostylisITS_v1.3_dryad.nex
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Abstract
Morphological and molecular phylogenetic evidence indicate that Eleutherostylis Burret (Malvaceae: Grewioideae), a monotypic genus described from New Guinea, is best considered a synonym of Grewia L., a species-rich genus widespread across the paleotropics and found in Africa, Arabia, Asia, Australia, and the western Pacific. A new combination based on E. renistipulata Burret, G. renistipulata (Burret) Dorr, comb. nov., is proposed. Original material of the basionym could not be located and a neotype is designated. A lectotype is designated for G. morotaiensis Kosterm., a synonym of G. renistipulata.
README: Reevaluating monotypic Eleutherostylis from New Guinea and the Moluccas, and its inclusion in Grewia (Malvaceae: Grewioideae)
This DNA dataset, based on nuclear ribosomal Internal Transcribed Spacer (ITS), is designed to reconstruct phylogenetic relationships of Eleutherostylis (Malvaceae) and is derived from fluorescent Sanger sequencing on an ABI 3730xl DNA Analyzer. The reads were assembled with Sequencher v5.2.4 and consensus sequences were aligned with MAFFT v.7.272 (Katoh and Standley 2013) under the G-INS-i refinement method followed by minor manual refinements based on a similarity criterion.
Description of the data and file structure
This dataset is in the NEXUS file format with commands designed to be executed in PAUP*, and matrix derivatives then analyzed with other phylogenetic analysis programs. The dataset has pseudo-phylogenetic taxon order and a 119 bp deletion set (masking) is specified in the matrix which must be removed from brackets if implemented by the Exclude command. The published tree included all data and the masking was only used in a preliminary alignment-sensitivity analysis.
Sharing/Access information
All sequence data is publicly accessible in GenBank (https://www.ncbi.nlm.nih.gov/genbank/). GenBank accession numbers are published in the article associated this submission.
Methods
This DNA dataset, based on nuclear ribosomal Internal Transcribed Spacer (ITS), is designed to reconstruct phylogenetic relationships of Eleutherostylis (Malvaceae) and is derived from fluorescent Sanger sequencing on an ABI 3730xl DNA Analyzer. The reads were assembled with Sequencher v5.2.4 and consensus sequences were aligned with MAFFT v.7.272 (Katoh and Standley 2013) under the G-INS-i refinement method followed by minor manual refinements based on a similarity criterion.