Data from: Characterization of 42 polymorphic microsatellite loci in Mimulus ringens (Phrymaceae) using Illumina sequencing

Nunziata, Schyler O., University of Georgia

Karron, Jeffrey D., University of Wisconsin-Madison

Mitchell, Randall J., University of Akron

Lance, Stacey L., University of Georgia

Jones, Kenneth L., University of Colorado School of Medicine, 12801 E. 17th Ave., Aurora, Colorado 80045 USA

Trapnell, Dorset W., University of Georgia

Publication date: December 5, 2012

Publisher: Dryad

https://doi.org/10.5061/dryad.cr3g5v4f

Citation

Nunziata, Schyler O. et al. (2012), Data from: Characterization of 42 polymorphic microsatellite loci in Mimulus ringens (Phrymaceae) using Illumina sequencing, Dryad, Dataset, https://doi.org/10.5061/dryad.cr3g5v4f

Abstract

Premise of the study: Microsatellite markers were isolated and characterized in Mimulus ringens (Phrymaceae), a herbaceous wetland perennial, to facilitate studies of mating patterns and population genetic structure. Methods and Results: A total of 42 polymorphic loci were identified from a sample of 24 individuals from a single popula- tion in Ohio, USA. The number of alleles per locus ranged from two to nine, and median observed heterozygosity was 0.435. Conclusions: This large number of polymorphic loci will enable researchers to quantify male fitness, patterns of multiple pa- ternity, selfing, and biparental inbreeding in large natural populations of this species. These markers will also permit detailed study of fine-scale patterns of genetic structure.

Usage notes

Mimulus_msats

This is a zipped file that contains two fastq files. They represent the data for microsatellite loci developed for Mimulus ringens from using Illumina paired-end reads. The paired-end reads are in separate files and for each sequence the locus name is in the title with the prefix "Miri" for Mimulus ringens.