Phenotypic data for: Does size-selective harvesting erode adaptive potential to thermal stress?
Data files
Feb 20, 2024 version files 399.13 KB
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Mortality.csv
9.71 KB
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README.md
3.59 KB
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ThemralStressCTMax.csv
5.25 KB
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ThermalStressBehaviour.csv
11.67 KB
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ThermalStressEggData.csv
45.46 KB
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ThermalStressFecundity.csv
1.25 KB
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ThermalStressFeedingBehaviour.csv
7.52 KB
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ThermalStressLengthWeight.csv
299.85 KB
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ThermalStressMetabolic.csv
14.83 KB
Abstract
Overharvesting is a serious threat to many fish populations. High mortality and directional selection on body size can cause evolutionary change in exploited populations, via selection for a specific phenotype and a potential reduction in phenotypic diversity. Whether the loss of phenotypic diversity that accompanies directional selection impairs response to environmental stress is not known. To address this question, we exposed three zebrafish selection lines to thermal stress. Two lines had experienced directional selection for (1) large and (2) small body size, and one was (3) subject to random removal of individuals with respect to body size (i.e. line with no directional selection). Selection lines were exposed to three temperatures (elevated, 34°C; ambient, 28°C; low, 22°C) to determine the response to an environmental stressor (thermal stress). We assessed differences among selection lines in their life history (growth and reproduction), physiological traits (metabolic rate and critical thermal max), and behaviour (activity and feeding behaviour) when reared at different temperatures. Lines experiencing directional selection (i.e., size selected) showed reduced growth rate and a shift in average phenotype in response to lower or elevated thermal stress compared with fish from the random-selected line. Our data indicate that populations exposed to directional selection can have a more limited capacity to respond to thermal stress compared with fish that experience a comparable reduction in population size (but without directional selection). Future studies should aim to understand the impacts of environmental stressors on natural fish stocks.
README: Phenotypic data for paper entitled: Does size-selective harvesting erode adaptive potential to thermal stress?
https://doi.org/10.5061/dryad.cvdncjt9v
Data for phenotypic data from thermal experiment, includes life history, physiological and behavioural data
Description of the data and file structure
Excel files of data used in experiment:
NA= no data
CTmax = Thermal maximum experiment
-Run = run number of experiment, Box= acclimation box, Rearing_Temperature = experimental rearing temperature (°C), Temperature = CTmax, Observer = who observed experiment, Date = date of experiment, Selection line = line replicate, Treat = pooled line replicate, Scope = Aerobic scope (°C), Cage = cage replicate, Col = colour of tag
LengthWeight = Growth rate data
-pic = picture ID, ID = replicate number, Tank = tank replicate, Line = line replicate, Selection = pooled line replicate, Rep = inividual replicate, colour = colour of tag, T = temperature (°C), Week = week, weight = weight (g), Temperature = temperature (°C), Length = length (mm), Cage = cage replicate, rep_ID = replicate
Behaviour = Activity, boldness and exploration
-Tank = tank replicate, Line = pooled line replicate, Cage = cage replicate, Temp = temperature (°C), Rep1 = replicate, image_top = image name of top camera, image_side = image number side camera, date = date of experiment, exp_tank, tank replicate of experiment, colour = colour of tag, number of time emerged = number of time emerged, Time emerged =first time emergence (s) , Total time emerged = total time spent emerged (s), Time per emerge = average time spent emerged (s), Selection = pooled line replicate
Feeding behaviour- feeding behaviour experiment
-Line=pooled line replicate, rep =replicate, selection = line replicate, Temp = temperature (°C), Num_feed = number of feeds, Time_to_feed = time taken till first feed (s), Feed_Cat= time categroy (s), Fed =whether fish eats or doesnt, colour=colour of tag, Tank= tank replicate, cage= cage replicate, weight = weight (g)
Metabolic = SMR, MMR, AAS
-Photo = photo number, Length = length of fish (mm), Date = date of experiment, Time = time period of experiment, Temp = temperature (°C), Tank = tank replicate, Cage = cage replicate, Treat = pooled line replicate, Line = line replicate, Chamber.Respirometer = chamber replicate number, Weight = weight of fish (g), SMR = standard metabolic rate (mgO2 g−1 h−1), MMR = maximum metabolic rate (mgO2 g−1 h−1), r2 = r2 value, color = colour of tag
Mortality = Deaths throughout experiment
-Tank = tank replicate, Line = line replicate, Selection = pooled line replicate, Rep = replicate, Colour = tag colour, T = temperature (°C), Mortality = death or no death, Cage = cage replicate
Fecundity = egg number
-Line = line replicate, Selection = pooled line replicate, Rep_line = replicate, Box = breeding box, Fertilised = number of fertilised eggs, Unfertilised = number of unfertilised eggs, Total viable = fertlised + unfertilised eggs, Dead = dead eggs, Temp = temperature (°C), Date = date of experiment
Egg data = Metrics used for eggs/larvae
-Treat = pooled line replicate, Line = line replicate, Rep = replicate of spawning tank, Date = date of experiment, Well = well number on breeding plate, Egg(L) = egg diameter (um), Yolk(L) = yolk diameter (um), Larvae(L)= larvae length (um), LarvaeYolk(L) = larvae yolk diameter(um), Hatchdate= date of hatching, Pic = picture number, Temperature = temperature (°C), Hatchday = days until hatch, Hatched = hatched or not hatched, Mothers = mother ID.
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