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Data from: The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications

Citation

Bui, Khanh Huy et al. (2020), Data from: The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications, Dryad, Dataset, https://doi.org/10.5061/dryad.d51c59zxt

Abstract

Microtubules are cytoskeletal structures involved in stability, transport and organization in the cell. The building blocks, the α- and β-tubulin heterodimers, form protofilaments that associate laterally into the hollow microtubule. Microtubule also exists as highly stable doublet microtubules in the cilia where stability is needed for ciliary beating and function. The doublet microtubule maintains its stability through interactions at its inner and outer junctions where its A- and B-tubules meet. Here, using cryo-electron microscopy, bioinformatics and mass spectrometry of the doublets of Chlamydomonas reinhardtii and Tetrahymena thermophila, we identified two new inner junction proteins, FAP276 and FAP106, and an inner junction-associated protein, FAP126, thus presenting the complete answer to the inner junction identity and localization. Our structural study of the doublets shows that the inner junction serves as an interaction hub that involves tubulin post-translational modifications. These interactions contribute to the stability of the doublet and hence, normal ciliary motility.

Methods

Cilia were purified from Chlamydomonas, salt-treated to remove outside proteins. The samples were subjected to tandem mass spectrometry according to the protocol described in Dai et al. 2019, bioRxiv. (https://doi.org/10.1101/739383)

Funding

Natural Sciences and Engineering Research Council of Canada, Award: RGPIN-2016-04954

Canadian Institutes of Health Research, Award: PJT-156354

Natural Sciences and Engineering Research Council of Canada, Award: RGPIN-2018-04813

Natural Sciences and Engineering Research Council of Canada, Award: RGPIN-2017-04649

Canadian Institute for Advanced Research, Award: Arzieli Global Scholar Program

Japan Society for the Promotion of Science

Canada Research Chairs, Award: 950-229792

Abdulla Al Ghurair Foundation for Education, Award: Al Ghurair STEM program scholarship