https://doi.org/10.5061/dryad.d51c5b0bn

Description of the data and file structure

COMBINED_NGS_SANGER.phy

This is the PHYLIP formatted concatenated alignment of 2,291 loci (totaling 1,803,947 bp) used as input for phylogenetic analysis in our study. These sequences were derived from several sources including hybrid capture (Anchored Hybrid Enrichment - AHE), OrthoMCL mining of low-coverage whole genomes, and Sanger sequencing of 3 nuclear ribosomal loci (18S, 28S D2-3, 28S D3-5) and 2 mitochondrial genes (16S and CO1). The AHE and OrthoMCL portions of the concatenated alignments in this file (totaling 2,286 loci) were processed and first analyzed by Knyshov et al. 2023 (https://doi.org/10.1093/molbev/msad168). See Supplementary Table 1 of our paper for more information on how sequence data was obtained for each voucher specimen and a list of accession numbers for raw Sanger sequences available on GenBank. Note: some taxon names in this file may differ from those presented in the figures of our paper; tip_names.csv provides a key that links original taxon names in the matrix to the updated names presented in the figures.

NGS_SANGER_MORPH.best_models.nex

This NEXUS formatted file provides partition position information for the 2,291 molecular loci in our concatenated alignment (COMBINED_NGS_SANGER.phy) and our morphological dataset (Reduviidae_morph_matrix.phy) as well as the best corresponding substitution models for each partition (as determined using ModelFinder in IQ-TREE). This file was used for initiation of phylogenetic reconstruction in IQ-TREE. These are specific commands we used to run the analysis: iqtree2 -s COMBINED_NGS_SANGER.phy -p NGS_SANGER_MORPH.best_models.nex -st DNA  –alrt 1000 -B 1000 -allnni -pre iqtree -safe -T AUTO

Combined_molecular_data_partitions.prt

This file provides a map to the individual molecular partitions and their positions in our concatenated DNA alignment (COMBINED_NGS_SANGER.phy). 2,293 partitions are listed because COI is divided into 1st, 2nd, and 3rd codon positions. This file can be used to deconstruct the concatenated matrix into individual aligment files per locus if necessary.

Reduviidae_morph_matrix.nex / Reduviidae_morph_matrix.phy

These are NEXUS and PHYLIP formatted versions of our morphological matrix consisting of 112 characters coded for 200 taxa. In total, 34 head, 20 thoracic, 16 leg, 11 forewing, and 25 abdominal characters were scored using Mesquite v3.70. Images of select characters and their states are provided in figures 2–5 of our paper. The .phy version was used in conjunction with the molecular concatenated alignment (COMBINED_NGS_SANGER.phy) to perform a combined morphological + molecular analysis in IQ-TREE. The NEXUS formatted version provides more details and lists all of the character state codings for 112 morphological characters that we surveyed. Note: some taxon names in this file may differ from those presented in the figures of our paper; tip_names.csv provides a key that links original taxon names in the matrix to the updated names presented in the figures.

tip_names.csv

This file provides a key that links original taxon names in the matrixes/raw tree files to the updated names presented in the figures. The original name for each voucher used in our matrixes is given in the first column. The updated generic-level names and species epithets for each voucher applied in the figures are listed in columns “genus” and “species”. Biogeographic regions (“locality” column) corresponding to each specimen are provided as AT=Afrotropical, AU=Australian, NE=Nearctic, NT=Neotropical, OT=Oriental, PA=Palearctic. Lab specific unique specimen identifiers are listed in column “RCW”. N/As in the spreadsheet represent not applicable information (these occur among non-reduviid outgroup taxa).

ASTRAL_tree.txt

This is the raw species tree recovered from the ASTRAL analysis of the molecular dataset. Local posterior probability values are listed across internal nodes. Note: some taxon names in this file may differ from those presented in the figures of our paper; tip_names.csv provides a key that links original taxon names in the matrix to the updated names presented in the figures.

ML_tree.txt

This is the best Maximum Likelihood tree (of eight independent runs) recovered from the IQ-TREE analysis of our combined morphological (Reduviidae_morph_matrix.phy) + molecular (COMBINED_NGS_SANGER.phy) datasets. SH-aLRT branch test and Ultra-Fast bootstrap approximation support values are listed across internal nodes. Note: some taxon names in this file may differ from those presented in the figures of our paper; tip_names.csv provides a key that links original taxon names in the matrix to the updated names presented in the figures.

ML_tree_for_ASR.tre

This file contains the same ML tree (ML_tree.txt) recovered using IQ-TREE, but with updated taxon names that reflect those used in the main figures and the ancestral state reconstructions. This file was used in conjunction with morph_matrix_for_ASR.csv morphological matrix file to preform ASR with the ‘ace’ function of the package ape.

morph_matrix_for_ASR.csv

This .csv contains the morphological matrix as formatted for ancestral state reconstruction in R. The 200 taxa we coded are listed in the first column. The 112 discrete characters (starting with “Char_0…”) are listed in the first row. Each cell in the matrix details all of the individual character states per taxon. This file was used in conjunction with ML_tree_for_ASR.tre tree file to preform ASR with the ‘ace’ function of the package ape.