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Data from: Long-lived marine species may be resilient to environmental variability through a temporal portfolio effect

Citation

Maselko, Jacek; Andrews, Kimberly; Hohenlohe, Paul (2021), Data from: Long-lived marine species may be resilient to environmental variability through a temporal portfolio effect, Dryad, Dataset, https://doi.org/10.5061/dryad.d7wm37pz7

Abstract

Maintenance of a portfolio of adaptive alleles may provide resilience of populations to natural environmental variability. We used Pacific ocean perch (POP; Sebastes alutus) to test for the maintenance of adaptive variation across overlapping generations. POP are a long-lived species characterized by widespread larval dispersal in their first year and a longevity of over 100 years. In order to understand how early marine dispersal affects POP survival and population structure, we used Restriction Site Associated DNA sequencing (RADseq) to obtain 11,146 single-nucleotide polymorphisms (SNPs) from 401 young-of-the-year (YOY) POP collected during surveys conducted in 2014 (19 stations) and 2015 (4 stations) in the eastern Gulf of Alaska. Population clustering analysis showed that the POP samples represented four distinct ancestral populations mixed throughout the sampling area. Based on prior work on larval dispersal of POP, these larvae are most likely from distinct parturition locations that are mixing during their pelagic dispersal life stage. Latent factor mixed models revealed that POP larvae face significant selection during their first year at sea, which were specific to the year of their birth. Thus each adult cohort’s genetic composition is heavily influenced by the environmental conditions experienced during their first year at sea. Long-lived species relying on broadcast spawning strategies may therefore be uniquely resilient to environmental variability by maintaining a portfolio of cohort-specific adaptive genotypes, and age truncation due to overfishing of older cohorts may have detrimental effect on the population viability.

Methods

Young-of-the-year Pacific ocean perch (Sebastes alutus) were collected during NOAA oceanographic surveys in the summer of 2014 (July 8 to August 14) and 2015 (July 13 to July 22). The larvae and young of the year fish were distinguished from congenerics using diagnostic SNPs prior to inclusion in this study. The resulting sample size of identified YOY POP was 399 fish in 2014 and 108 in 2015.

Fish length, weight, and lipid content for each identified POP fish were measured at Auke Bay Laboratories in Juneau, AK.  For DNA analysis, a small tissue plug was extracted through an incision in the abdominal wall that included the heart tissue and stored in 95% ethanol.

RADseq library preparation was done for 513 samples including eight samples that were replicates using the Sbf1 restriction enzyme and 150 bp paired end sequencing was done on two lanes using Illumina HiSeq 4000.

Usage Notes

Note that the prepared sample size and the recovered sample size are slightly different due to no DNA recovered in a couple of plate wells.

Funding

National Institute of General Medical Sciences, Award: P30 GM103324

NOAA Research

University of Idaho