Data from: Green derivative synchronous spectrofluorimetry for kinetic stability study of florfenicol
Data files
May 31, 2024 version files 188.27 KB
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Original_data.docx
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README.md
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Abstract
This dataset contains data representing synchronous fluorescence spectra obtained upon utilizing spectrofluorometry as a highly sensitive analysis tool for the simultaneous determination of Florfenicol with its different degradation products obtained after stress testing. Synchronous fluorimetry was used followed by applying either first or second derivatization according to the type of degradation aiming to obtain highly resoluted spectra. Different degradation pathways according to published literature were used to interpret degradation kinetic parameters, which were in accordance with previously reported articles. The dataset also includes different validation parameters applied.
https://doi.org/10.5061/dryad.dfn2z358b
Description of the data and file structure
| F1 | Scanning of 10.0 µg/mL at ∆ƛ=20 nm |
|---|---|
| F2 | Scanning of 10.0 µg/mL at ∆ƛ=40 nm |
| F3 | Scanning of 10.0 µg/mL at ∆ƛ=60 nm |
| F4 | Scanning of 10.0 µg/mL at ∆ƛ=80 nm |
| F5 | Scanning of 10.0 µg/mL at ∆ƛ=100 nm |
| F6 | Scanning of 10.0 µg/mL at ∆ƛ=120 nm |
| F7 | 0.5µg/mL FLR |
| F8 | 2.0 µg/mL FLR |
| F9 | 4.0 µg/mL FLR |
| F10 | 6.0 µg/mL FLR |
| F11 | 12.0 µg/mL FLR |
| F12 | 16.0 µg/mL FLR |
| F13 | Alkaline degradation of 10.0 µg/mL FLR using 1M NaOH at 100̊C at ∆ƛ=20 |
| F14 | Alkaline degradation of 10.0 µg/mL FLR at ∆ƛ= 40 using 1M NaOH at 100̊C |
| F15 | Alkaline degradation of 10.0 µg/mL FLR at ∆ƛ= 60 using 1M NaOH at 100̊C |
| F16 | Alkaline degradation of 10.0 µg/mL FLR at ∆ƛ= 80 using 1M NaOH at 100̊C |
| F17 | Alkaline degradation of 10.0 µg/mL FLR at ∆ƛ= 100 using 1M NaOH at 100̊C |
| F18 | Alkaline degradation of 10.0 µg/mL FLR at ∆ƛ= 120 using 1M NaOH at 100̊C |
| F19 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 40̊C after 5 minutes using 1M NaOH |
| F20 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 40̊C after 10 minutes using 1M NaOH |
| F21 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 40̊C after 20 minutes using 1M NaOH |
| F22 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 40̊C after 25 minutes using 1M NaOH |
| F23 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 40̊C after 30 minutes using 1M NaOH |
| F24 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 40̊C after 45 minutes using 1M NaOH |
| F25 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 40̊C after 60 minutes using 1M NaOH |
| F26 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 5 minutes using 1M NaOH |
| F27 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 10 minutes using 1M NaOH |
| F28 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 15 minutes using 1M NaOH |
| F29 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 20 minutes using 1M NaOH |
| F30 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 25 minutes using 1M NaOH |
| F31 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 30 minutes using 1M NaOH |
| F32 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 45 minutes using 1M NaOH |
| F33 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 60 minutes using 1M NaOH |
| F34 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 60̊C after 90 minutes using 1M NaOH |
| F35 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 80̊C after 5 minutes using 1M NaOH |
| F36 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 80̊C after 10 minutes using 1M NaOH |
| F37 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 80̊C after 20 minutes using 1M NaOH |
| F38 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 80̊C after 25 minutes using 1M NaOH |
| F39 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 80̊C after 30 minutes using 1M NaOH |
| F40 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 80̊C after 45 minutes using 1M NaOH |
| F41 | Kinetic alkaline degradation of 10.0 µg/mL FLR at 80̊C after 60 minutes using 1M NaOH |
| F42 | Alkaline degradation product of 10.0 µg/mL FLR at 100̊C after 5 minutes using 1M NaOH |
| F43 | Alkaline degradation product of 10.0 µg/mL FLR at 100̊C after 15 minutes using 1M NaOH |
| F44 | Alkaline degradation product of 10.0 µg/mL FLR at 100̊C after 20 minutes using 1M NaOH |
| F45 | Alkaline degradation product of 10.0 µg/mL FLR at 100̊C after 25 minutes using 1M NaOH |
| F46 | Alkaline degradation product of 10.0 µg/mL FLR at 100̊C after 30 minutes using 1M NaOH |
| F47 | Alkaline degradation product of 10.0 µg/mL FLR at 100̊C after 60 minutes using 1M NaOH |
| F48 | Alkaline degradation product of 10.0 µg/mL FLR at 100̊C after 90 minutes using 1M NaOH |
| F49 | Acidic degradation product of 10.0 µg/mL FLR after 5 min at 40̊C using 1M HCl |
| F50 | Acidic degradation product of 10.0 µg/mL FLR after 10 min at 40̊C using 1M HCl |
| F51 | Acidic degradation product of 10.0 µg/mL FLR after 15 min at 40̊C using 1M HCl |
| F52 | Acidic degradation product of 10.0 µg/mL FLR after 20 min at 40̊C using 1M HC |
| F53 | Acidic degradation product of 10.0 µg/mL FLR after 25 min at 40̊C using 1M HCl |
| F54 | Acidic degradation product of 10.0 µg/mL FLR after 90 min at 40̊C using 1M HCl |
| F55 | Acidic degradation product of 10.0 µg/mL FLR after 5 min at 60̊ C using 1M HCl |
| F56 | Acidic degradation product of 10.0 µg/mL FLR after 10 min at 60̊ C using 1M HCl |
| F57 | Acidic degradation product of 10.0 µg/mL FLR after 15 min at 60̊ C using 1M HCl |
| F58 | Acidic degradation product of 10.0 µg/mL FLR after 25 min at 60̊ C using 1M HCl |
| F59 | Acidic degradation product of 10.0 µg/mL FLR after 45 min at 60̊ C using 1M HCl |
| F60 | Acidic degradation product of 10.0 µg/mL FLR after 60 min at 60̊ C using 1M HCl |
| F61 | Acidic degradation product of 10.0 µg/mL FLR after 90 min at 60̊ C using 1M HCl |
| F62 | Acidic degradation product of 10.0 µg/mL FLR after 5 min at 80̊ C using 1M HCl |
| F63 | Acidic degradation product of 10.0 µg/mL FLR after 10 min at 80̊ C using 1M HCl |
| F64 | Acidic degradation product of 10.0 µg/mL FLR after 15 min at 80̊ C using 1M HCl |
| F65 | Acidic degradation product of 10.0 µg/mL FLR after 20 min at 80̊ C using 1M HCl |
| F66 | Acidic degradation product of 10.0 µg/mL FLR after 30 min at 80̊ C using 1M HCl |
| F67 | Acidic degradation product of 10.0 µg/mL FLR after 60 min at 80̊ C using 1M HCl |
| F68 | Acidic degradation product of 10.0 µg/mL FLR after 90 min at 80̊ C using 1M HCl |
| F69 | Acidic degradation product of 10.0 µg/mL FLR after 5 min at 100̊C using 1M HCl |
| F70 | Acidic degradation product of 10.0 µg/mL FLR after 10 min at 100̊C using 1M HCl |
| F71 | Acidic degradation product of 10.0 µg/mL FLR after 15 min at 100̊C using 1M HCl |
| F72 | Acidic degradation product of 10.0 µg/mL FLR after 45 min at 100̊C using 1M HCl |
| F73 | Acidic degradation product of 10.0 µg/mL FLR after 90 min at 100̊C using 1M HCl |
| F74 | Oxidative degradation product of 10.0µg/mL after 5 min using 0.07%H2O2 at 40̊C |
| F75 | Oxidative degradation product of 10.0µg/mL after 15 min using 0.07%H2O2 at 40̊C |
| F76 | Oxidative degradation product of 10.0µg/mL after 30 min using 0.07%H2O2 at 40̊C |
| F77 | Oxidative degradation product of 10.0µg/mL after 5 min using 0.07%H2O2 at 100̊C |
| F78 | Oxidative degradation product of 10.0µg/mL after 30 min using 0.07%H2O2 at 100̊C |
| F79 | Scanning of oxidative degradation product of 10.0 µg/mL using 0.07% H2O2 at room temperature at ∆ƛ=20 nm |
| F80 | Scanning of oxidative degradation product of 10.0 µg/mL using 0.07% H2O2 at room temperature at ∆ƛ=40 nm |
| F81 | Scanning of oxidative degradation product of 10.0 µg/mL using 0.07% H2O2 at room temperature at ∆ƛ=60 nm |
| F82 | Scanning of oxidative degradation product of 10.0 µg/mL using 0.07% H2O2 at room temperature at ∆ƛ=80 nm |
| F83 | Scanning of oxidative degradation product of 10.0 µg/mL using 0.07% H2O2 at room temperature at ∆ƛ=100 nm |
| F84 | Scanning of oxidative degradation product of 10.0 µg/mL using 0.07% H2O2 at room temperature at ∆ƛ=120 nm |
| F85 | Scanning of UV degradation product of 10.0µg/mL FLR at ∆ƛ=20 nm |
| F86 | Scanning of UV degradation product of 10.0µg/mL FLR at ∆ƛ=40 nm |
| F87 | Scanning of UV degradation product of 10.0µg/mL FLR at ∆ƛ=60 nm |
| F88 | Scanning of UV degradation product of 10.0µg/mL FLR at ∆ƛ=80 nm |
| F89 | Scanning of UV degradation product of 10.0µg/mL FLR at ∆ƛ=100 nm |
| F90 | Scanning of UV degradation product of 10.0µg/mL FLR at ∆ƛ=120 nm |
The selected synchronous fluorescence spectra obtained during forced degradation of FLR. Information on how the mathematical processing was performed to calculate different kinetic data and validation parameters can be found in the associated manuscript referenced above.
The dataset was collected during the measurement of fluorescence following the acidic, alkaline, oxidative, and photolytic degradation of FLR at different temperature settings at predetermined time intervals. Labsolution software was used to apply synchronous fluorimetry at different Δλ, followed by either first or second derivatization. The experiments were carried out in the Central Laboratory at the Faculty of Pharmacy Delta University for Science and Technology. The synchronous fluorescence spectra illustrated in the following table are the result of measuring the synchronous fluorescence relative intensity on the Y axis versus the emission wavelength on the X axis.