Data for: Whole brain in situ mapping of neuronal activation in Drosophila during social behaviors and optogenetic stimulation
Data files
Oct 30, 2024 version files 99.36 MB
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Hr38_data_for_deposit.zip
99.36 MB
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README.md
654 B
Abstract
Monitoring neuronal activity at single-cell resolution in freely moving Drosophila engaged in social behaviors is challenging because of their small size and lack of transparency. Extant methods, such as Flyception, are highly invasive. Whole-brain calcium imaging in head-fixed, walking flies is feasible but the animals cannot perform the consummatory phases of social behaviors like aggression or mating under these conditions. This has left open the fundamental question of whether neurons identified as functionally important for such behaviors using loss- or gain-of-function screens are actually active during the natural performance of such behaviors, and if so during which phase(s). Here we perform brain-wide mapping of active cells expressing the Immediate Early Gene hr38 using a high-sensitivity/low background FISH amplification method called HCR-3.0. Using double-labeling for hr38 mRNA and for GFP, we describe the activity of several classes of aggression-promoting neurons during courtship and aggression, including P1a cells, an intensively studied population of male-specific interneurons. Using HI-FISH in combination with optogenetic activation of aggression-promoting neurons (opto-HI-FISH) we identify candidate downstream functional targets of these cells in a brain-wide, unbiased manner. Finally, we compare the activity of P1a neurons during sequential performance of courtship and aggression, using intronic vs. exonic hr38 probes to differentiate newly synthesized nuclear transcripts from cytoplasmic transcripts synthesized at an earlier time. These data provide evidence suggesting that different subsets of P1a neurons may be active during courtship vs. aggression. HI-FISH and associated methods may help to fill an important lacuna in the armamentarium of tools for neural circuit analysis in Drosophila.
README: Data for: Whole brain in situ mapping of neuronal activation in Drosophila during social behaviors and optogenetic stimulation
https://doi.org/10.5061/dryad.dr7sqvb7d
Description of the data and file structure
The zip file contains confocal images and calcium imaging data.
Files and variables
File: Hr38_data_for_deposit.zip
Description:
The folder structure is organized by figure numbers, with subfolders corresponding to figure panels used in the associated manuscript.
Most confocal images are in 16-bit grayscale TIFF format.
The data includes unmerged and uncropped images