Raw Raman data of Mycobacterium bovis BCG, Rhodococcus erythropolis, & Corynebacterium glutamicum
Data files
Sep 21, 2023 version files 18.16 MB
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C._Glutamicum__3_0__X_8199.22__Y_14523__Time_0.txt
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C._Glutamicum__3_1__X_8204.22__Y_14523__Time_50.txt
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C._Glutamicum__3_10__X_8199.22__Y_14528__Time_509.txt
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C._Glutamicum__3_11__X_8204.22__Y_14528__Time_560.txt
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C._Glutamicum__3_12__X_8209.22__Y_14528__Time_611.txt
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C._Glutamicum__3_13__X_8214.22__Y_14528__Time_661.txt
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C._Glutamicum__3_14__X_8219.22__Y_14528__Time_712.txt
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C._Glutamicum__3_15__X_8224.22__Y_14528__Time_763.txt
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C._Glutamicum__3_16__X_8229.22__Y_14528__Time_814.txt
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C._Glutamicum__3_17__X_8234.22__Y_14528__Time_865.txt
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C._Glutamicum__3_18__X_8239.22__Y_14528__Time_916.txt
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C._Glutamicum__3_19__X_8244.22__Y_14528__Time_967.txt
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C._Glutamicum__3_2__X_8209.22__Y_14523__Time_101.txt
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C._Glutamicum__3_20__X_8199.22__Y_14533__Time_1018.txt
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C._Glutamicum__3_21__X_8204.22__Y_14533__Time_1069.txt
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C._Glutamicum__3_22__X_8209.22__Y_14533__Time_1120.txt
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C._Glutamicum__3_23__X_8214.22__Y_14533__Time_1171.txt
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C._Glutamicum__3_24__X_8219.22__Y_14533__Time_1222.txt
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C._Glutamicum__3_25__X_8224.22__Y_14533__Time_1273.txt
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C._Glutamicum__3_26__X_8229.22__Y_14533__Time_1323.txt
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C._Glutamicum__3_27__X_8234.22__Y_14533__Time_1374.txt
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C._Glutamicum__3_28__X_8239.22__Y_14533__Time_1425.txt
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C._Glutamicum__3_29__X_8244.22__Y_14533__Time_1476.txt
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C._Glutamicum__3_3__X_8214.22__Y_14523__Time_152.txt
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C._Glutamicum__3_30__X_8199.22__Y_14538__Time_1527.txt
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C._Glutamicum__3_31__X_8204.22__Y_14538__Time_1578.txt
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C._Glutamicum__3_32__X_8209.22__Y_14538__Time_1629.txt
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C._Glutamicum__3_33__X_8214.22__Y_14538__Time_1680.txt
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C._Glutamicum__3_34__X_8219.22__Y_14538__Time_1731.txt
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C._Glutamicum__3_35__X_8224.22__Y_14538__Time_1782.txt
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C._Glutamicum__3_36__X_8229.22__Y_14538__Time_1833.txt
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C._Glutamicum__3_37__X_8234.22__Y_14538__Time_1884.txt
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C._Glutamicum__3_38__X_8239.22__Y_14538__Time_1935.txt
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C._Glutamicum__3_39__X_8244.22__Y_14538__Time_1985.txt
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C._Glutamicum__3_4__X_8219.22__Y_14523__Time_203.txt
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C._Glutamicum__3_40__X_8199.22__Y_14543__Time_2036.txt
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C._Glutamicum__3_41__X_8204.22__Y_14543__Time_2087.txt
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C._Glutamicum__3_42__X_8209.22__Y_14543__Time_2138.txt
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C._Glutamicum__3_43__X_8214.22__Y_14543__Time_2189.txt
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C._Glutamicum__3_44__X_8219.22__Y_14543__Time_2240.txt
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C._Glutamicum__3_45__X_8224.22__Y_14543__Time_2291.txt
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C._Glutamicum__3_46__X_8229.22__Y_14543__Time_2342.txt
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C._Glutamicum__3_47__X_8234.22__Y_14543__Time_2393.txt
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C._Glutamicum__3_48__X_8239.22__Y_14543__Time_2444.txt
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C._Glutamicum__3_49__X_8244.22__Y_14543__Time_2495.txt
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C._Glutamicum__3_5__X_8224.22__Y_14523__Time_254.txt
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C._Glutamicum__3_50__X_8199.22__Y_14548__Time_2546.txt
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C._Glutamicum__3_51__X_8204.22__Y_14548__Time_2597.txt
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C._Glutamicum__3_52__X_8209.22__Y_14548__Time_2647.txt
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C._Glutamicum__3_53__X_8214.22__Y_14548__Time_2698.txt
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C._Glutamicum__3_54__X_8219.22__Y_14548__Time_2749.txt
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C._Glutamicum__3_55__X_8224.22__Y_14548__Time_2800.txt
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C._Glutamicum__3_56__X_8229.22__Y_14548__Time_2851.txt
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C._Glutamicum__3_57__X_8234.22__Y_14548__Time_2902.txt
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C._Glutamicum__3_58__X_8239.22__Y_14548__Time_2953.txt
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C._Glutamicum__3_59__X_8244.22__Y_14548__Time_3004.txt
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C._Glutamicum__3_6__X_8229.22__Y_14523__Time_305.txt
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C._Glutamicum__3_60__X_8199.22__Y_14553__Time_3055.txt
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C._Glutamicum__3_61__X_8204.22__Y_14553__Time_3106.txt
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C._Glutamicum__3_62__X_8209.22__Y_14553__Time_3157.txt
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C._Glutamicum__3_63__X_8214.22__Y_14553__Time_3208.txt
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C._Glutamicum__3_64__X_8219.22__Y_14553__Time_3259.txt
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C._Glutamicum__3_65__X_8224.22__Y_14553__Time_3310.txt
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C._Glutamicum__3_66__X_8229.22__Y_14553__Time_3360.txt
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C._Glutamicum__3_67__X_8234.22__Y_14553__Time_3411.txt
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C._Glutamicum__3_68__X_8239.22__Y_14553__Time_3462.txt
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C._Glutamicum__3_69__X_8244.22__Y_14553__Time_3513.txt
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C._Glutamicum__3_7__X_8234.22__Y_14523__Time_356.txt
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C._Glutamicum__3_70__X_8199.22__Y_14558__Time_3564.txt
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C._Glutamicum__3_71__X_8204.22__Y_14558__Time_3615.txt
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C._Glutamicum__3_72__X_8209.22__Y_14558__Time_3666.txt
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C._Glutamicum__3_73__X_8214.22__Y_14558__Time_3717.txt
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C._Glutamicum__3_74__X_8219.22__Y_14558__Time_3768.txt
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C._Glutamicum__3_75__X_8224.22__Y_14558__Time_3819.txt
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C._Glutamicum__3_76__X_8229.22__Y_14558__Time_3870.txt
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C._Glutamicum__3_77__X_8234.22__Y_14558__Time_3920.txt
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C._Glutamicum__3_78__X_8239.22__Y_14558__Time_3971.txt
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C._Glutamicum__3_79__X_8244.22__Y_14558__Time_4022.txt
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C._Glutamicum__3_8__X_8239.22__Y_14523__Time_407.txt
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C._Glutamicum__3_80__X_8199.22__Y_14563__Time_4073.txt
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C._Glutamicum__3_81__X_8204.22__Y_14563__Time_4124.txt
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C._Glutamicum__3_82__X_8209.22__Y_14563__Time_4175.txt
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C._Glutamicum__3_83__X_8214.22__Y_14563__Time_4226.txt
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C._Glutamicum__3_84__X_8219.22__Y_14563__Time_4277.txt
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C._Glutamicum__3_85__X_8224.22__Y_14563__Time_4328.txt
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C._Glutamicum__3_86__X_8229.22__Y_14563__Time_4379.txt
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C._Glutamicum__3_87__X_8234.22__Y_14563__Time_4430.txt
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C._Glutamicum__3_88__X_8239.22__Y_14563__Time_4481.txt
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C._Glutamicum__3_89__X_8244.22__Y_14563__Time_4532.txt
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C._Glutamicum__3_9__X_8244.22__Y_14523__Time_458.txt
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C._Glutamicum__3_90__X_8199.22__Y_14568__Time_4583.txt
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C._Glutamicum__3_91__X_8204.22__Y_14568__Time_4633.txt
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C._Glutamicum__3_92__X_8209.22__Y_14568__Time_4684.txt
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C._Glutamicum__3_93__X_8214.22__Y_14568__Time_4735.txt
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C._Glutamicum__3_94__X_8219.22__Y_14568__Time_4786.txt
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C._Glutamicum__3_95__X_8224.22__Y_14568__Time_4837.txt
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C._Glutamicum__3_96__X_8229.22__Y_14568__Time_4888.txt
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C._Glutamicum__3_97__X_8234.22__Y_14568__Time_4939.txt
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C._Glutamicum__3_98__X_8239.22__Y_14568__Time_4990.txt
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C._Glutamicum__3_99__X_8244.22__Y_14568__Time_5041.txt
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C.Glutamicum_785nm_11.06.2021_0__X_-29055.8__Y_-37360.8__Time_0.txt
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C.Glutamicum_785nm_11.06.2021_1__X_-29050.8__Y_-37360.8__Time_50.txt
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C.Glutamicum_785nm_11.06.2021_10__X_-29055.8__Y_-37355.8__Time_506.txt
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C.Glutamicum_785nm_11.06.2021_11__X_-29050.8__Y_-37355.8__Time_556.txt
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C.Glutamicum_785nm_11.06.2021_12__X_-29045.8__Y_-37355.8__Time_607.txt
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C.Glutamicum_785nm_11.06.2021_13__X_-29040.8__Y_-37355.8__Time_657.txt
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C.Glutamicum_785nm_11.06.2021_14__X_-29035.8__Y_-37355.8__Time_708.txt
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C.Glutamicum_785nm_11.06.2021_15__X_-29030.8__Y_-37355.8__Time_758.txt
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C.Glutamicum_785nm_11.06.2021_16__X_-29025.8__Y_-37355.8__Time_809.txt
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C.Glutamicum_785nm_11.06.2021_17__X_-29020.8__Y_-37355.8__Time_860.txt
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C.Glutamicum_785nm_11.06.2021_18__X_-29015.8__Y_-37355.8__Time_910.txt
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C.Glutamicum_785nm_11.06.2021_19__X_-29010.8__Y_-37355.8__Time_961.txt
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C.Glutamicum_785nm_11.06.2021_2__X_-29045.8__Y_-37360.8__Time_101.txt
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C.Glutamicum_785nm_11.06.2021_20__X_-29055.8__Y_-37350.8__Time_1012.txt
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C.Glutamicum_785nm_11.06.2021_21__X_-29050.8__Y_-37350.8__Time_1062.txt
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C.Glutamicum_785nm_11.06.2021_22__X_-29045.8__Y_-37350.8__Time_1113.txt
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C.Glutamicum_785nm_11.06.2021_23__X_-29040.8__Y_-37350.8__Time_1163.txt
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C.Glutamicum_785nm_11.06.2021_24__X_-29035.8__Y_-37350.8__Time_1214.txt
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C.Glutamicum_785nm_11.06.2021_25__X_-29030.8__Y_-37350.8__Time_1264.txt
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C.Glutamicum_785nm_11.06.2021_26__X_-29025.8__Y_-37350.8__Time_1315.txt
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C.Glutamicum_785nm_11.06.2021_27__X_-29020.8__Y_-37350.8__Time_1366.txt
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C.Glutamicum_785nm_11.06.2021_28__X_-29015.8__Y_-37350.8__Time_1416.txt
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C.Glutamicum_785nm_11.06.2021_29__X_-29010.8__Y_-37350.8__Time_1467.txt
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C.Glutamicum_785nm_11.06.2021_3__X_-29040.8__Y_-37360.8__Time_151.txt
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C.Glutamicum_785nm_11.06.2021_30__X_-29055.8__Y_-37345.8__Time_1518.txt
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C.Glutamicum_785nm_11.06.2021_31__X_-29050.8__Y_-37345.8__Time_1568.txt
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C.Glutamicum_785nm_11.06.2021_32__X_-29045.8__Y_-37345.8__Time_1619.txt
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C.Glutamicum_785nm_11.06.2021_33__X_-29040.8__Y_-37345.8__Time_1669.txt
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C.Glutamicum_785nm_11.06.2021_34__X_-29035.8__Y_-37345.8__Time_1720.txt
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C.Glutamicum_785nm_11.06.2021_35__X_-29030.8__Y_-37345.8__Time_1771.txt
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C.Glutamicum_785nm_11.06.2021_36__X_-29025.8__Y_-37345.8__Time_1821.txt
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C.Glutamicum_785nm_11.06.2021_37__X_-29020.8__Y_-37345.8__Time_1872.txt
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C.Glutamicum_785nm_11.06.2021_38__X_-29015.8__Y_-37345.8__Time_1922.txt
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C.Glutamicum_785nm_11.06.2021_39__X_-29010.8__Y_-37345.8__Time_1973.txt
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C.Glutamicum_785nm_11.06.2021_4__X_-29035.8__Y_-37360.8__Time_202.txt
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C.Glutamicum_785nm_11.06.2021_40__X_-29055.8__Y_-37340.8__Time_2024.txt
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C.Glutamicum_785nm_11.06.2021_41__X_-29050.8__Y_-37340.8__Time_2074.txt
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C.Glutamicum_785nm_11.06.2021_42__X_-29045.8__Y_-37340.8__Time_2125.txt
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C.Glutamicum_785nm_11.06.2021_43__X_-29040.8__Y_-37340.8__Time_2175.txt
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C.Glutamicum_785nm_11.06.2021_44__X_-29035.8__Y_-37340.8__Time_2226.txt
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C.Glutamicum_785nm_11.06.2021_45__X_-29030.8__Y_-37340.8__Time_2277.txt
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C.Glutamicum_785nm_11.06.2021_46__X_-29025.8__Y_-37340.8__Time_2327.txt
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C.Glutamicum_785nm_11.06.2021_47__X_-29020.8__Y_-37340.8__Time_2378.txt
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C.Glutamicum_785nm_11.06.2021_48__X_-29015.8__Y_-37340.8__Time_2428.txt
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C.Glutamicum_785nm_11.06.2021_49__X_-29010.8__Y_-37340.8__Time_2479.txt
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C.Glutamicum_785nm_11.06.2021_5__X_-29030.8__Y_-37360.8__Time_252.txt
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C.Glutamicum_785nm_11.06.2021_50__X_-29055.8__Y_-37335.8__Time_2530.txt
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C.Glutamicum_785nm_11.06.2021_51__X_-29050.8__Y_-37335.8__Time_2580.txt
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C.Glutamicum_785nm_11.06.2021_52__X_-29045.8__Y_-37335.8__Time_2631.txt
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C.Glutamicum_785nm_11.06.2021_53__X_-29040.8__Y_-37335.8__Time_2682.txt
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C.Glutamicum_785nm_11.06.2021_54__X_-29035.8__Y_-37335.8__Time_2732.txt
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C.Glutamicum_785nm_11.06.2021_55__X_-29030.8__Y_-37335.8__Time_2783.txt
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C.Glutamicum_785nm_11.06.2021_56__X_-29025.8__Y_-37335.8__Time_2833.txt
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C.Glutamicum_785nm_11.06.2021_57__X_-29020.8__Y_-37335.8__Time_2884.txt
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C.Glutamicum_785nm_11.06.2021_58__X_-29015.8__Y_-37335.8__Time_2934.txt
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C.Glutamicum_785nm_11.06.2021_59__X_-29010.8__Y_-37335.8__Time_2985.txt
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C.Glutamicum_785nm_11.06.2021_6__X_-29025.8__Y_-37360.8__Time_303.txt
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C.Glutamicum_785nm_11.06.2021_60__X_-29055.8__Y_-37330.8__Time_3036.txt
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C.Glutamicum_785nm_11.06.2021_61__X_-29050.8__Y_-37330.8__Time_3086.txt
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C.Glutamicum_785nm_11.06.2021_62__X_-29045.8__Y_-37330.8__Time_3137.txt
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C.Glutamicum_785nm_11.06.2021_63__X_-29040.8__Y_-37330.8__Time_3188.txt
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C.Glutamicum_785nm_11.06.2021_64__X_-29035.8__Y_-37330.8__Time_3238.txt
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C.Glutamicum_785nm_11.06.2021_65__X_-29030.8__Y_-37330.8__Time_3289.txt
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C.Glutamicum_785nm_11.06.2021_66__X_-29025.8__Y_-37330.8__Time_3339.txt
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C.Glutamicum_785nm_11.06.2021_67__X_-29020.8__Y_-37330.8__Time_3390.txt
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C.Glutamicum_785nm_11.06.2021_68__X_-29015.8__Y_-37330.8__Time_3440.txt
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C.Glutamicum_785nm_11.06.2021_69__X_-29010.8__Y_-37330.8__Time_3491.txt
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C.Glutamicum_785nm_11.06.2021_7__X_-29020.8__Y_-37360.8__Time_353.txt
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C.Glutamicum_785nm_11.06.2021_70__X_-29055.8__Y_-37325.8__Time_3542.txt
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C.Glutamicum_785nm_11.06.2021_71__X_-29050.8__Y_-37325.8__Time_3593.txt
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C.Glutamicum_785nm_11.06.2021_72__X_-29045.8__Y_-37325.8__Time_3643.txt
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C.Glutamicum_785nm_11.06.2021_73__X_-29040.8__Y_-37325.8__Time_3694.txt
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C.Glutamicum_785nm_11.06.2021_74__X_-29035.8__Y_-37325.8__Time_3744.txt
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C.Glutamicum_785nm_11.06.2021_75__X_-29030.8__Y_-37325.8__Time_3795.txt
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C.Glutamicum_785nm_11.06.2021_76__X_-29025.8__Y_-37325.8__Time_3845.txt
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C.Glutamicum_785nm_11.06.2021_77__X_-29020.8__Y_-37325.8__Time_3896.txt
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C.Glutamicum_785nm_11.06.2021_78__X_-29015.8__Y_-37325.8__Time_3947.txt
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C.Glutamicum_785nm_11.06.2021_79__X_-29010.8__Y_-37325.8__Time_3997.txt
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C.Glutamicum_785nm_11.06.2021_8__X_-29015.8__Y_-37360.8__Time_404.txt
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C.Glutamicum_785nm_11.06.2021_80__X_-29055.8__Y_-37320.8__Time_4048.txt
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C.Glutamicum_785nm_11.06.2021_81__X_-29050.8__Y_-37320.8__Time_4099.txt
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C.Glutamicum_785nm_11.06.2021_82__X_-29045.8__Y_-37320.8__Time_4149.txt
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C.Glutamicum_785nm_11.06.2021_83__X_-29040.8__Y_-37320.8__Time_4200.txt
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C.Glutamicum_785nm_11.06.2021_84__X_-29035.8__Y_-37320.8__Time_4250.txt
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C.Glutamicum_785nm_11.06.2021_85__X_-29030.8__Y_-37320.8__Time_4301.txt
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C.Glutamicum_785nm_11.06.2021_86__X_-29025.8__Y_-37320.8__Time_4351.txt
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C.Glutamicum_785nm_11.06.2021_87__X_-29020.8__Y_-37320.8__Time_4402.txt
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C.Glutamicum_785nm_11.06.2021_88__X_-29015.8__Y_-37320.8__Time_4453.txt
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C.Glutamicum_785nm_11.06.2021_89__X_-29010.8__Y_-37320.8__Time_4503.txt
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C.Glutamicum_785nm_11.06.2021_9__X_-29010.8__Y_-37360.8__Time_455.txt
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C.Glutamicum_785nm_11.06.2021_90__X_-29055.8__Y_-37315.8__Time_4554.txt
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C.Glutamicum_785nm_11.06.2021_91__X_-29050.8__Y_-37315.8__Time_4605.txt
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C.Glutamicum_785nm_11.06.2021_92__X_-29045.8__Y_-37315.8__Time_4655.txt
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C.Glutamicum_785nm_11.06.2021_93__X_-29040.8__Y_-37315.8__Time_4706.txt
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C.Glutamicum_785nm_11.06.2021_94__X_-29035.8__Y_-37315.8__Time_4756.txt
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C.Glutamicum_785nm_11.06.2021_95__X_-29030.8__Y_-37315.8__Time_4807.txt
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C.Glutamicum_785nm_11.06.2021_96__X_-29025.8__Y_-37315.8__Time_4858.txt
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C.Glutamicum_785nm_11.06.2021_97__X_-29020.8__Y_-37315.8__Time_4908.txt
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C.Glutamicum_785nm_11.06.2021_98__X_-29015.8__Y_-37315.8__Time_4959.txt
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C.Glutamicum_785nm_11.06.2021_99__X_-29010.8__Y_-37315.8__Time_5009.txt
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C.glutimacum_785nm_06.02.2020_0__X_1800.36__Y_-26651.8__Time_0.txt
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C.glutimacum_785nm_06.02.2020_1__X_1805.36__Y_-26651.8__Time_50.txt
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C.glutimacum_785nm_06.02.2020_10__X_1800.36__Y_-26646.8__Time_506.txt
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C.glutimacum_785nm_06.02.2020_11__X_1805.36__Y_-26646.8__Time_556.txt
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C.glutimacum_785nm_06.02.2020_12__X_1810.36__Y_-26646.8__Time_607.txt
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C.glutimacum_785nm_06.02.2020_13__X_1815.36__Y_-26646.8__Time_658.txt
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C.glutimacum_785nm_06.02.2020_14__X_1820.36__Y_-26646.8__Time_708.txt
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C.glutimacum_785nm_06.02.2020_15__X_1825.36__Y_-26646.8__Time_759.txt
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README.md
Abstract
Even in the face of the COVID-19 pandemic, Tuberculosis (TB) continues to be a major public health problem and the 2nd biggest infectious cause of death worldwide. There is, therefore, an urgent need to develop effective TB diagnostic methods, which are cheap, portable, sensitive and specific. Raman spectroscopy is a potential spectroscopic technique for this purpose, however, so far, research efforts have focused primarily on the characterisation of Mycobacterium tuberculosis and other Mycobacteria, neglecting bacteria within the microbiome and thus, failing to consider the bigger picture. It is paramount to characterise relevant Mycobacteriales and develop suitable analytical tools to discriminate them from each other. Herein, through the combined use of Raman spectroscopy and the self-optimising Kohonen index network and further multivariate tools, we have successfully undertaken the spectral analysis of Mycobacterium bovis BCG, Corynebacterium glutamicum and Rhodoccocus erythropolis. This has led to development of a useful tool set, which can readily discern spectral differences between these three closely related bacteria as well as generate a unique spectral barcode for each species. Further optimisation and refinement of the developed method will enable its application to other bacteria inhabiting the microbiome and ultimately lead to advanced diagnostic technologies, which can save many lives.
Methods
Spectral acquisitions were performed using an InVia confocal Raman (Renishaw). The spectrometer was calibrated prior to each use with silicon (520.7cm-1 ). A 100x Leica objective and a 1200l/mm grating were used for all measurements in the range of 750-1750cm-1 . Raman map scans, consisting of 3 accumulations and 10 second exposure time, were acquired over a 10µm x 10µm square grid using a 785nm excitation laser with laser power of 10-14mW. 100 spectra were collected for each bacterium with 3 replicas, generating a total of n=300 spectra per bacterial species. Wire 5.1 (Renishaw Plc) software was used for baseline subtraction and cosmic ray removal.
Files were then exported in .txt format.
Usage notes
Notepad