Maintaining the integrity of the genome is of utmost importance for cell division and propagation. In Escherichia coli, the RecG protein has been implicated in processing branched recombination intermediates during DNA repair processes, but the primary cellular role(s) of RecG and the repair pathways in which it acts have been difficult to define. To gain additional insight into RecG function, we employed transposon sequencing (Tn-seq) to identify recG genetic interactions and reveal complementary or redundant functions. The strongest hits from the screen were the dam, uvrD, rnhA, radA, and rep genes. The conditional importance of these five genes in cells lacking recG was confirmed using a plasmid-based assay, revealing synthetic lethal interactions for most double deletion strains. Several of the synthetic lethal gene combinations (with uvrD, rep, and radA; but not rnhA or dam) were suppressed by deletion of recF or recO, indicating that their genetic relationships involved roles in post-replication gap repair. Additionally, loss of the RecG/SSB interaction phenocopied a recG deletion when combined with dam, uvrD, radA, or rnhA deletions but not with rep. The results reinforce the idea of RecG as a general genome guardian. Its varied genetic interactions reflect critical roles in several different repair contexts, where RecG alleviates toxic DNA intermediates.

Can use MochiView of IGV to view gene insertion profiles.