Data from: Variations in HLA-B cell surface expression, half-life and extracellular antigen receptivity
Data files
Jul 10, 2019 version files 48.54 GB
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Beads.zip
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Donor 1 - Bw6.zip
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Donor 102 - Bw6.zip
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Donor 106 - Bw4.zip
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Donor 111 - Bw4:Bw6.zip
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Donor 112 - Bw4.zip
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Donor 115 - Bw6.zip
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Donor 119 - Bw4.zip
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Donor 12 - Bw4:Bw6.zip
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Donor 120 - Bw6.zip
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Donor 121 - Bw4:Bw6.zip
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Donor 124 - Bw6.zip
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Donor 126 - Bw4.zip
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Donor 128 - Bw4:Bw6.zip
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Donor 130 - Bw4-Bw6.zip
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Donor 131 - Bw6.zip
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Donor 136 - Bw4.zip
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Donor 137 - Bw4:Bw6.zip
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Donor 137 peptide receptivity.zip
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Donor 14 - Bw4:Bw6.zip
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Donor 14 peptide receptivity.zip
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Donor 141 - Bw4:Bw6.zip
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Donor 141 peptide receptivity.zip
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Donor 142 - Bw4.zip
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Donor 143 - Bw4.zip
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Donor 155 - Bw4.zip
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Donor 156 - Bw4.zip
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Donor 166 - Bw6.zip
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Donor 168 - Bw4:Bw6.zip
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Donor 17 - Bw6.zip
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Donor 178 - Bw4:Bw6.zip
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Donor 178 peptide receptivity.zip
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Donor 187 - Bw4:Bw6.zip
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Donor 187 peptide receptivity.zip
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Donor 194 - Bw4:Bw6.zip
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Donor 196 - Bw6.zip
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Donor 198 - Bw4:Bw6.zip
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Donor 198 peptide receptivity.zip
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Donor 20 - Bw4:Bw6.zip
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Donor 201 - Bw6.zip
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Donor 202 - Bw4.zip
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Donor 206 - Bw6.zip
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Donor 23 - Bw4.zip
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Donor 237 ImageStream.zip
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Donor 24 -Bw6.zip
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Donor 24 peptide receptivity.zip
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Donor 26 - Bw6.zip
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Donor 28 - Bw4:Bw6 .zip
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Donor 28 peptide receptivity.zip
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Donor 31 - Bw6.zip
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Donor 31 peptide receptivity.zip
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Donor 34 - Bw6.zip
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Donor 35 - Bw6.zip
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Donor 5 - Bw4:Bw6.zip
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Donor 55 - Bw6.zip
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Donor 57 - Bw6.zip
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Donor 62 - Bw4.zip
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Donor 64 ImageStream.zip
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Donor 64 peptide receptivity.zip
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Donor 71 - Bw6.zip
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Donor 71 peptide receptivity.zip
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Donor 75 - Bw4:Bw6.zip
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Donor 79 - Bw4:Bw6.zip
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Donor 8 - Bw4:Bw6.zip
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Donor 80 - Bw4:Bw6.zip
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Donor 9 - Bw6 .zip
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Donor 91 - Bw4:Bw6.zip
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Donor 94 - Bw4:Bw6 .zip
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Donor 94 ImageStream.zip
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Donor 94 peptide receptivity.zip
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Donor111.zip
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Donor120.zip
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Donor121.zip
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Donor124.zip
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Donor126.zip
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Donor128.zip
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Donor131.zip
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Donor137_Part1.zip
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Donor137_Part2.zip
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Donor137_Part3.zip
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Donor14.zip
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Donor141.zip
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Donor142.zip
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Donor155.zip
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Donor156.zip
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Donor178_Part1.zip
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Donor178_Part2.zip
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Donor187_Part1.zip
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Donor187_Part2.zip
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Donor194.zip
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Donor198.zip
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Donor20.zip
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Donor206.zip
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Donor215.zip
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Donor24_Part1.zip
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Donor24_Part2.zip
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Donor24_Part3.zip
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Donor28_Part1.zip
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Donor28_Part2.zip
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Donor31_Part1.zip
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Donor31_Part2.zip
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Donor31_Part3.zip
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Donor55.zip
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Donor62.zip
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Donor64_Part1.zip
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Donor64_Part2.zip
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Donor64_Part3.zip
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Donor71.zip
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Donor8.zip
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Donor80.zip
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Donor91.zip
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Donor94_Part1.zip
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Donor94_Part2.zip
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dryad.f0s23r8 README.rtf
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ShannonEntropy.zip
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W6-32_Tapasin_Intracellular-Staining.zip
Abstract
The highly polymorphic human leukocyte antigen (HLA) class I molecules present peptide antigens to CD8+ T cells, inducing immunity against infections and cancers. Quality control mediated by peptide loading complex (PLC) components is expected to ensure the cell surface expression of stable peptide-HLA class I complexes. This is exemplified by HLA-B*08:01 in primary human lymphocytes, with both expression level and half-life at the high end of the measured HLA-B expression and stability hierarchies. Conversely, low expression on lymphocytes is measured for three HLA-B allotypes that bind peptides with proline at position 2, which are disfavored by the transporter associated with antigen processing. Surprisingly, these lymphocyte-specific expression and stability differences become reversed or altered in monocytes, which display larger intracellular pools of HLA class I than lymphocytes. Together, the findings indicate that allele and cell-dependent variations in antigen acquisition pathways influence HLA-B surface expression levels, half-lives and receptivity to exogenous antigens.