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Data from: Resurrected “ancient” Daphnia genotypes show reduced thermal stress tolerance compared to modern descendants

Citation

Yousey, Aimee M. et al. (2018), Data from: Resurrected “ancient” Daphnia genotypes show reduced thermal stress tolerance compared to modern descendants, Dryad, Dataset, https://doi.org/10.5061/dryad.f30r1

Abstract

Understanding how populations adapt to rising temperatures has been a challenge in ecology. Research often evaluates multiple populations to test whether local adaptation to temperature regimes is occurring. Space-for-time substitutions are common, as temporal constraints limit our ability to observe evolutionary responses. We employed a resurrection ecology approach to understand how thermal tolerance has changed in a Daphnia pulicaria population over time. Temperatures experienced by the oldest genotypes were considerably lower than the youngest. We hypothesized clones were adapted to the thermal regimes of their respective time periods. We performed two thermal shock experiments that varied in length of heat exposure. Overall trends revealed that younger genotypes exhibited higher thermal tolerance than older genotypes; heat shock protein (hsp70) expression increased with temperature and varied among genotypes, but not across time periods. Our results indicate temperature may have been a selective factor on this population, although the observed responses may be a function of multifarious selection. Prior work found striking changes in population genetic structure, and in other traits that were strongly correlated with anthropogenic changes. Resurrection ecology approaches should help our understanding of interactive effects of anthropogenic alterations to temperature and other stressors on the evolutionary fate of natural populations.

Usage Notes

Funding

National Science Foundation, Award: U.S. National Science Foundation (NSF-IOS-OEI) collaborative grants #0924289 and #1256881 to Lawrence J. Weider and Grant #0924401 to Punidan Jeyasingh.

Location

South Center Lake Minnesota