Activation of autophagy during normothermic machine perfusion of discarded livers is associated with improved hepatocellular function

Ohman, Anders, Brown University, https://orcid.org/0000-0002-3146-9448

Raigani, Siavash, Massachusetts General Hospital, https://orcid.org/0000-0003-2762-7287

Santiago, John, Brown University

Heaney, Megan, Brown University

Boylan, Joan, Brown University

Parry, Nicola, Tufts University

Carroll, Cailah, Massachusetts General Hospital

Baptista, Sofia, Massachusetts General Hospital

Uygun, Korkut, Massachusetts General Hospital

Gruppuso, Phillip, Brown University

Sanders, Jennifer, Brown University

Yeh, Heidi, Massachusetts General Hospital

Santiago, John C., Rhode Island Hospital

Heaney, Megan G., Rhode Island Hospital

Boylan, Joan M., Rhode Island Hospital

Baptista, Sofia G., Massachusetts General Hospital

Gruppuso, Philip A., Rhode Island Hospital

Sanders, Jennifer A., Rhode Island Hospital

anders_ohman@brown.edu

Published Oct 26, 2021 on Dryad. https://doi.org/10.5061/dryad.f7m0cfxx0

Cite this dataset

Ohman, Anders et al. (2021). Activation of autophagy during normothermic machine perfusion of discarded livers is associated with improved hepatocellular function [Dataset]. Dryad. https://doi.org/10.5061/dryad.f7m0cfxx0

Abstract

We demonstrate that ischemia-reperfusion injury occurs in all livers during NMP, though there are notable differences in gene expression between functional and nonfunctional livers. We further demonstrate that activation of the liver’s repair and homeostasis mechanisms through autophagy plays a vital role in the graft’s response to injury and may impact liver function. These findings indicate that liver autophagy might be a key therapeutic target for rehabilitating the function of severely injured or untransplantable livers.

Funding

National Institute of Environmental Health Sciences, Award: T32ES007272

Massachusetts General Hospital Executive Committee on Research

National Institutes of Diabetes and Digestive and Kidney Diseases, Award: R01DK096075, R01DK107875, R01DK114506

National Science Foundation, Award: EEC 1941543, ATP-Bio

Rhode Island Hospital/Brown University Department of Pediatrics

HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases, Award: R01DK096075

HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases, Award: R01DK107875

HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases, Award: R01DK114506

HHS | NIH | National Institute of Environmental Health Sciences, Award: T32ES007272

National Science Foundation, Award: EEC 1941543