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The oxytocin-prostaglandins pathways in the horse (Equus caballus) placenta during pregnancy, physiological parturition, and parturition with fetal membrane retention

Citation

Rapacz-Leonard, Anna et al. (2020), The oxytocin-prostaglandins pathways in the horse (Equus caballus) placenta during pregnancy, physiological parturition, and parturition with fetal membrane retention, Dryad, Dataset, https://doi.org/10.5061/dryad.fbg79cnr4

Abstract

Despite their importance in mammalian reproduction, substances in the oxytocin-prostaglandins pathways have not been investigated in the horse placenta during most of pregnancy and parturition. Therefore, we quantified placental content of oxytocin (OXT), oxytocin receptor (OXTR), and prostaglandin E2 and F2 alpha during days 90–240 of pregnancy (PREG), physiological parturition (PHYS), and parturition with fetal membrane retention (FMR) in heavy draft horses (PREG = 13, PHYS = 11, FMR = 10). We also quantified OXTR and prostaglandin endoperoxide synthase-2 (PTGS2) mRNA expression and determined the immunolocalization of OXT, OXTR, and PTGS2. For relative quantification of OXT and OXTR, we used western blotting with densitometry. To quantify the prostaglandins, we used enzyme immunoassays. For relative quantification of OXTR and PTGS2, we used RT-qPCR. For immunolocalization of OXT, OXTR, and PTGS2, we used immunohistochemistry. We found that OXT was present in cells of the allantochorion and endometrium in all groups. PTGS2 expression in the allantochorion was 14.7-fold lower in FMR than in PHYS (p = 0.007). These results suggest that OXT is synthesized in the horse placenta. As PTGS2 synthesis is induced by inflammation, they also suggest that FMR in heavy draft horses may be associated with dysregulation of inflammatory processes.

Methods

For relative quantification of mRNA, we used RT-qPCR. For relative quantification of oxytocin peptide and oxytocin receptor protein, we used western blotting with densitometry. To quantify prostaglandin E2 and PGF content, we used enzyme immunoassays.

Usage Notes

This dataset contains two files (please download both of them):

-‘Rapacz-Leonard_data.csv’ contains the data

-‘Rapacz-Leonard_script.txt’ contains instructions and the script for replicating the statistical analyses 

Required packages for R:
To use the script in 'Rapacz-Leonard_script.txt' file, you will need to have the following packages installed:
- the tidyverse family of packages (can be installed with command install.packages("tidyverse"))
- WRS (download the Rallfun file from the author's website, https://dornsife.usc.edu/labs/rwilcox/software/)

Funding

Narodowe Centrum Nauki, Award: 2012/07/D/NZ5/04290