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Size-dependent predation and intraspecific inhibition of an estuarine snail feeding on oysters

Citation

Pusack, Tim et al. (2020), Size-dependent predation and intraspecific inhibition of an estuarine snail feeding on oysters, Dryad, Dataset, https://doi.org/10.5061/dryad.ffbg79crw

Abstract

Predator outbreaks have increased in the past two decades in many ecosystems and are predicted to become more common with climate change. During these outbreaks, predator densities increase rapidly, and can cause large reductions in prey populations or shifts in prey size structure. However, unexpected interactions may occur at high predator densities, necessitating a mechanistic understanding of how increased predator density affects predator-prey dynamics. In the northern Gulf of Mexico, outbreaks of southern oyster drill (Stramonita haemastoma, Linnaeus, 1767) occur during high salinity events, and can greatly reduce eastern oyster (Crassostrea virginica, Gmelin, 1791) populations. A large outbreak of drills occurred from 2013—2015 in Apalachicola Bay, FL which corresponded with an oyster fishery collapse in that bay. To improve our mechanistic understanding of predation during such a high-density outbreak, laboratory experiments based on field observations were used to quantify the prey size selection by drills and the drill functional response, as a function of drill abundance. Drills fed on medium-sized oysters (50-75 mm) more often than small and larger-sized oysters, and often formed aggregations during feeding events. However, despite this aggregative response, there was a negative relationship between per capita feeding rates and drill abundance. Indeed, the Crowley-Martin functional response model had the most parsimonious fit to the data, suggesting that predator-predator inhibition reduced attack rates and increased handling times. Due to an increase in regional drought conditions and water usage in the southeastern United States, drill outbreaks will likely increase in frequency and duration. A greater understanding of how predation rates change with predator densities during outbreaks will improve predictions of oyster mortality, and strengthen the scientific framework for oyster fishery decisions.

Methods

This experiment used drills and oysters that were collected from subtidal habitats in Apalachicola Bay, FL USA (both collections occurred around 29º 40’ 32.56” N, 84º 51’ 36.76” W and 29º 42’ 7.2” N, 84º 49’ 31.76” W), and was carried out at the University of South Florida, College of Marine Science in St. Petersburg, FL (USFCMS). At USFCMS three separate, closed-seawater systems, each comprising ten experimental tanks (47.3 l; 68 ´ 40 ´ 27.5 cm), were constructed for a total of 30 tanks. The base area of these tanks was similar to the first experiment, 0.27 m2. Within each system, approximately 950 l of artificial seawater was recirculated through the experimental tanks and a large sump (633 l; 76 ´ 45.5 ´ 183 cm). In Experiment II at UFSCMS, salinity was maintained at 25 by mixing deionized water with Instant Ocean sea salt (Instant Ocean Spectrum Brands, Blacksburg, VA, USA), and checked daily with a YSI 85 (Xylem Inc, Yellow Springs, OH, USA). Water temperature was maintained between 20-22°C using Finnex TH-800 Plus, 800-watt titanium heating rods (Finnex, Chicago IL, USA) with a Reef Octopus Heater Controller (Honya Co. Ltd, Shenzhen, China). The temperature and salinity were chosen based on conditions that occurred during an outbreak in the northern GOM (Menzel et al.,1966; FFWCC, 2013). During both experiments and the one week holding prior to Experiment II, oysters were fed Instant Algae Shellfish Diet 1800 (Reed Mariculture Inc, San Jose, CA) daily, following the manufacturer’s instructions of 3.6 ml per 100 g of oyster wet weight.

In the seawater system at USFCMS, six drill abundances (2, 3, 4, 6, 8, and 12 drills per tank) were orthogonally crossed with five oyster abundances (3, 4, 6, 8, and 12 per tank), yielding two replicates for each orthogonal cross. The wide range of abundances reflects a response-surface approach to the regression analysis, improving the chances of detecting interactions between experimental factors despite low replication at any one combination of treatments. The treatment abundances were equivalent to densities ranging from 7-44 m-2 (drills) and 16-65 m-2 (oysters), both within the natural range observed for each species during and after outbreak conditions (See Supplemental Fig. 1). In this second experiment, drills ranged in size from 30-81 mm and were separated into small (30-55 mm) and large (56-81 mm) size classes. Oysters ranged in size from 25-99 mm and were separated into small (25-49 mm), medium (50-74 mm), and large (75-99 mm) size classes, as in Experiment I. Abundances of drills and oysters were split equally among each size class. To quantify oyster mortality in the absence of drills, each level of oyster abundance was paired with a corresponding control treatment (n=2) that lacked an oyster drill; no oysters died in these controls. After collection from the field, drills were held for one week and allowed to feed ad libitum on oysters before the start of the experiment to reduce any transportation effect to USFCMS from Apalachicola Bay, FL. The experiment began with a 5-day starvation period, based on the feeding rate of individual-housed drills from Experiment I, to standardize predator hunger. During feeding trials, tanks were checked twice daily for dead oysters, and the number of drills on each oyster was counted. Any dead oysters were replaced with a live oyster from the same size class to preserve a constant prey density, as assumed in predator functional response models that quantify the instantaneous feeding rate as opposed to the integrated feeding rate. After the 15-day experimental period, both the per capita feeding rate and the aggregate feeding rate (oysters consumed per replicate) were calculated.

Usage Notes

Data descriptions for Functional Response Data from Pusack et al. 2018 JEMBE (501) 74-82

File: "drill aggregation feeding_ Table 1 Fig 5.csv"

Columns and descriptions:
round: the experiment was conducted in three rounds due to logistical constraints of the number of tanks available, not all treatments were done in each round so blocks were incomplete
tank: Tank ID per round for each treatment combination of drill and oyster abundance
drill.abundance: the number of drills placed in each tank
oyster.abundnace: the number of oysters maintained throughout the experiment. When an oyster was found dead, it was replaced with one of similar size.
total.no.oysters.consumed: over the entire experimental period, the total number of oysters consumed per tank
days: total length of the experimental period
oyster.per.drill: the number of oysters consumed per drill, which was calculated as = total.no.oyster.consumed/drill.abundnace
group.feeding.rate: the daily feeding rate for each tank, i.e. the group feeding rate (no. of oysters consumed/tank/day), which was calculated as = total.no.oysters.consumed/days
per.cap.feeding.rate: the per capita feeding rate for each tank (no. of oysters consumed/day/drill abundance of the tank), which was calculated as = total.no.oysters.consumed/days/drill.abundance

File: "drill size feeding Fig 1.csv"

Columns and descriptions:
 

column heading

description

round

Experiment number.  There were 2 round of this experiment

block

The experiment extended across several water tables.  These were considered blocks

water.depth

Whether the experiment took place in shallow or deep water

cage.no

There were several cages within each block.

drill.no

Drill identification.  There were 63 individual drills used in this experiment

drill.size.mm

Size of drill in mm.

drill.group

Drill size in small or large category (>59.5 is large, <59.5 is small)

date

Date the oyster was found dead.

day

Day of the experiment the oyster was found dead.

tday

Number of days the experiment lasted.

oys.size.mm

Size of the oyster in mm.

oys.type

Whether the oyster was present at the start of the experiment (original) or if it was replacement for a dead oyster during the middle of the experiment (replacement)

oys.status

Whether the oyster was a gaper or it was alive

gaper

whether the oyster was a gaper (1) or not (0).

drill.on.oyster

How many drills were found on the oyster?

oys.group

The sizes of oysters were split into groups (0-49=1, 50-74=2, 75+=3).

notes

Any extra notes.  Ex. was the oyster replaced? Were drill holes present?

File: "aggregation size and feeding Fig 2.csv"

Columns and descriptions:
drill.ttt: Number of drills in that tank

n: number of oysters observed over the course of the experiment

total: the total daily checks for each oyster when a feeding even could have occured

sub.total: a subtotal that limits the data to when a feeding event of at least 1 drill feeding on an oyster occured

oys.group: the number of drills in the feeding group on a single oyster

Values: whether the numbers are expected (exp) based on a poisson distribution or from observed (obs) data

prop.total: The proportion of the daily checks for each oyster with the number of drills (oys.group) feeding on an oyster. Zero, “0” is included in this total, i.e. no feeding.

prop.subtotal: The proportion of the specific oyster aggregation when a feeding event occurred (“0” or no feeding was excluded for this proporation).

values: the actual number observed of the feeding event of a specific drill aggregation size.

File: " drill aggregation feeding_ Fig 3 Fig 4.csv"

Columns and descriptions:

column heading

description

tank

There were 3 water tables each with 10 tanks (B3 refers to the third tank in the second water table.

drill.no.ttt

Number of drills in that tank

drill.den.m2

Density per meters^2 that this drill density in this tank would correspond to in nature

oys.no.ttt

Number of oysters in that tank

drill.oys.ttt

Combining the drill density and oyster density into a code for that tank

oys.den.m2

Density per meters^2 that the oyster density in this tank would correspond to in nature

mean.size

Mean size of the drills in that tank

sem.size

Standard error of the mean of the drills in that tank

tot.biomass

biomass in g of the drills in that tank

mean.biomass

mean biomass of the drills in that tank (tot.biomass/drill.no.ttt)

sem.biomass

standard error of the mean of biomass of drills in that tank

total

Total number of oysters eaten during the experiment

days

Number of days the experiment ran

per.ind

number of oysters eaten per drill (total/drill.no.ttt)

feed.rate

number of oysters eaten per day in that tank (total/days)

oys.day.ind

number of oysters eaten per day per drill in that tank (feed rate/drill.no.ttt)

Funding

Florida Department of Environmental Protection, Award: OGC071