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Dryad

NGS data from: Predominant HDR-dependent genome editing and prevention of byproducts by DNA repair pathway choice

Cite this dataset

Riesenberg, Stephan et al. (2023). NGS data from: Predominant HDR-dependent genome editing and prevention of byproducts by DNA repair pathway choice [Dataset]. Dryad. https://doi.org/10.5061/dryad.fj6q5740f

Abstract

CRISPR-Cas9 genome editing has been widely adapted to cleave genomic DNA sequences of interest. Cellular repair of the CRISPR induced DNA double-strand breaks is dominated by error prone end joining pathways that outcompete inefficient homology-directed repair (HDR) that would allow precise introduction of a mutation present in a supplied synthetic DNA donor. To achieve predominant HDR, we studied the combinatory effect of inhibitory mutations in genes that are central to competing repair pathways.

Funding

Max Planck Society

Nomis Foundation