Data from: Destabilizing mutations encode nongenetic variation that drives evolutionary innovation
Data files
Feb 02, 2019 version files 21.93 KB
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3-Mut_4-Jrev.seq
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3-Mut_7-Jrev.seq
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31F1_1a-J_Rev.ab1-1.fa
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4-MUT (P5_03-JRev).seq
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4-MUT (P5_04-JRev).seq
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5-MUT (Mage01_1-JRev).seq
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5-MUT (Mage01_2-JRev).seq
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Dryad Petrie Science 2018 Fig 1B and S1.csv
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Petrie Science 2018 Fig 2A.csv
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Petrie Science 2018 Fig 2B.csv
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Petrie Science 2018 Fig 2C.csv
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Petrie Science 2018 Fig 3A and 3B.csv
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Petrie Science 2018 Fig 3C.csv
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Petrie Science 2018 Fig 3D.csv
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Petrie Science 2018 Fig S4A and S4B.csv
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Petrie Science 2018 Fig S5.csv
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Petrie Science Fig 1C and S2.csv
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Abstract
Evolutionary innovations are often achieved by repurposing existing genes to perform new functions; however, the mechanisms enabling the transition from old to new remain controversial. We identified mutations in bacteriophage λ’s host-recognition gene J that confer enhanced adsorption to λ’s native receptor, LamB, and the ability to access a new receptor, OmpF. The mutations destabilize particles and cause conformational bistability of J, which yields progeny of multiple phenotypic forms, each proficient at different receptors. This work provides an example of how nongenetic protein variation can catalyze an evolutionary innovation. We propose that cases where a single genotype can manifest as multiple phenotypes may be more common than previously expected and offer a general mechanism for evolutionary innovation.