Data from: Na+/K+ pump interacts with the h-current to control bursting activity in central pattern generator neurons of leeches
Kueh, Daniel, Emory University
Barnett, William H., Georgia State University
Cymbalyuk, Gennady S., Georgia State University
Calabrese, Ronald L., Emory University
Published Aug 10, 2017 on Dryad.
Cite this dataset
Kueh, Daniel; Barnett, William H.; Cymbalyuk, Gennady S.; Calabrese, Ronald L. (2017). Data from: Na+/K+ pump interacts with the h-current to control bursting activity in central pattern generator neurons of leeches [Dataset]. Dryad. https://doi.org/10.5061/dryad.fr1r2
The dynamics of different ionic currents shape the bursting activity of neurons and networks that control motor output. Despite being ubiquitous in all animal cells, the contribution of the Na+/K+ pump current to such bursting activity has not been well studied. We used monensin, a Na+/H+ antiporter, to examine the role of the pump on the bursting activity of oscillator heart interneurons in leeches. When we stimulated the pump with monensin, the period of these neurons decreased significantly, an effect that was prevented or reversed when the h-current was blocked by Cs+. The decreased period could also occur if the pump was inhibited with strophanthidin or K+-free saline. Our monensin results were reproduced in model, which explains the pump’s contributions to bursting activity based on Na+ dynamics. Our results indicate that a dynamically oscillating pump current that interacts with the h-current can regulate the bursting activity of neurons and networks.
This archive contains the source for figure 7 A1 A2 A3 B2 B3.
In the directory B2_B3, the matlab script fixedIPump_withNa_JustHCO.m produces an eps file containing the figures found in 7B2 and 7B3.
In the directory A1_A2_A3, the matlab scrip trjHCO.m produces an eps file which contains the figures found in 7A1, 7A2, and 7A3.