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Two modes of gene regulation by TFL1 mediate its dual function in flowering time and shoot determinacy of Arabidopsis

Cerise, Martina1; Falavigna, Vitor da Silveira1; Maroto, Gabriel Rodriguez1; Signol, Antoine1; Severing, Edouard1; Gao, He1; Driel, Annabel van1; Vincent, Coral1; Wilkens, Sandra1; Iacobini, Francesca Romana1; Formosa-Jordan, Pau1; Pajoro, Alice2; Coupland, George1

Published Dec 01, 2023 on Dryad. https://doi.org/10.5061/dryad.fttdz090f

Data files

Dec 01, 2023 version files 115.75 GB

Abstract

Plant organ primordia develop successively at the shoot apical meristem (SAM). In Arabidopsis, primordia formed early in development differentiate into vegetative leaves, whereas those formed later generate inflorescence branches and flowers. TERMINAL FLOWER 1 (TFL1), a negative regulator of transcription, acts in the SAM to delay flowering and to maintain inflorescence meristem indeterminacy. We used confocal microscopy, time-resolved transcript profiling, and reverse genetics to elucidate this dual role of TFL1. We find that TFL1 accumulates dynamically in the SAM reflecting its dual function. Moreover, TFL1 represses two major sets of genes. One set includes genes that promote flowering and whose expression increases earlier in tfl1. The other set is spatially misexpressed in tfl1 inflorescence meristems. The misexpression of these two gene sets in tfl1 mutants depends upon FD transcription factor with which TFL1 interacts. Furthermore, the MADS-box gene SEPALLATA4, which is upregulated in tfl1, contributes both to the floral transition and shoot determinacy defects of tfl1 mutants. Thus, we delineate the dual function of TFL1 in shoot development in terms of its dynamic spatial distribution and different modes of gene repression.