Data from: Honeybees (Apis mellifera) learn color discriminations via differential conditioning independent of long wavelength (green) photoreceptor modulation
Reser, David H., Monash University
Witharanage Wijesekara, Randika, Monash University
Rosa, Marcello G. P., Monash University
Dyer, Adrian G., Monash University
Published Feb 22, 2013 on Dryad.
Cite this dataset
Reser, David H.; Witharanage Wijesekara, Randika; Rosa, Marcello G. P.; Dyer, Adrian G. (2013). Data from: Honeybees (Apis mellifera) learn color discriminations via differential conditioning independent of long wavelength (green) photoreceptor modulation [Dataset]. Dryad. https://doi.org/10.5061/dryad.g2r32
BACKGROUND: Recent studies on colour discrimination suggest that experience is an important factor in how a visual system processes spectral signals. In insects it has been shown that differential conditioning is important for processing fine colour discriminations. However, the visual system of many insects, including the honeybee, has a complex set of neural pathways, in which input from the long wavelength sensitive (‘green’) photoreceptor may be processed either as an independent achromatic signal or as part of a trichromatic opponent-colour system. Thus, a potential confound of colour learning in insects is the possibility that modulation of the ‘green’ photoreceptor could underlie observations. METHODOLOGY/PRINCIPAL FINDINGS: We tested honeybee vision using light emitting diodes centered on 414 and 424 nm wavelengths, which limit activation to the short-wavelength-sensitive (‘UV’) and medium-wavelength-sensitive (‘blue’) photoreceptors. The absolute irradiance spectra of stimuli was measured and modelled at both receptor and colour processing levels, and stimuli were then presented to the bees in a Y-maze at a large visual angle (26°), to ensure chromatic processing. Sixteen bees were trained over 50 trials, using either appetitive differential conditioning (N = 8), or aversive-appetitive differential conditioning (N = 8). In both cases the bees slowly learned to discriminate between the target and distractor with significantly better accuracy than would be expected by chance. Control experiments confirmed that changing stimulus intensity in transfers tests does not significantly affect bee performance, and it was possible to replicate previous findings that bees do not learn similar colour stimuli with absolute conditioning. CONCLUSION: Our data indicate that honeybee colour vision can be tuned to relatively small spectral differences, independent of ‘green’ photoreceptor contrast and brightness cues. We thus show that colour vision is at least partly experience dependent, and behavioural plasticity plays an important role in how bees exploit colour information.
Normal Bee data - spss output
Real text file containing touch test data in blocks of 5 trials
Quinine versus water 1
graph of quinine vs. H2O as aversive stimulus for differential conditioning.
graph pad Q versus water
Graphpad file containing intermediate and comparison data used to generate acquisition curves for differential conditioning procedure.
text file containing absolute irradiance data for 414 nm LED array obtained by Ocean Optics Spectrometer per methods.
Text file containing absolute irradiance data for 424 nm LED array. Note that output for both spectra files include wavelengths from 177.9 - 879.5 nm obtained at 75% of peak power supply output (details in methods).