Data from: Seasonal temperature, the lunar cycle and diurnal rhythms interact in a combinatorial manner to modulate genomic responses to the environment in a reef-building coral
Wuitchik, Daniel M.
Pells, Troy J.
Vize, Peter D.
Published Jul 01, 2019 on Dryad.
Cite this dataset
Wuitchik, Daniel M. et al. (2019). Data from: Seasonal temperature, the lunar cycle and diurnal rhythms interact in a combinatorial manner to modulate genomic responses to the environment in a reef-building coral [Dataset]. Dryad. https://doi.org/10.5061/dryad.g40g7k0
Rhythms of various periodicities drive cyclical processes in organisms ranging from single cells to the largest mammals on earth, and on scales from cellular physiology to global migrations. Molecular mechanisms that generate circadian behaviours in model organisms are well studied, but longer phase cycles and interactions between cycles with different periodicities are not well understood. Broadcast spawning corals are one of the best examples of an organism integrating inputs from multiple environmental parameters including seasonal temperature, the lunar phase, and hour of the day, to calibrate their annual reproductive event. We present a deep RNA-seq experiment utilizing multiple analyses to differentiate transcriptomic responses modulated by the interactions between the three aforementioned environmental parameters. Acropora millepora was sampled over multiple 24-hour periods throughout a full lunar month and at two seasonal temperatures. Temperature, lunar and diurnal cycles produce distinct transcriptomic responses, with interactions between all three variables identifying a core set of genes. These core genes include mef2, a developmental master regulator, and two hnRNPs, one of which is known to post-transcriptionally interact with mef2 and with biological clock regulating mRNAs. Interactions between diurnal and temperature differences impacted a range of core processes ranging from biological clocks to stress responses. Genes involved with developmental processes and transcriptional regulation were impacted by the lunar phase and seasonal temperature differences. Lastly, there was a diurnal and lunar phase interaction where genes involved with RNA-processing and translational regulation were differentially regulated. These data illustrate the extraordinary levels of transcriptional variation across time in a simple radial cnidarian in response to the environment under normal conditions.
The reference transcriptome used in the analysis. This was obtained from the laboratory of Misha Matz, UT Austin. This file is identical, except some fasta headers have had gene symbols updated. For the paper, isogroup numbers are the core identifier and isogroup to gene symbol mappings from the supplemental data should be used, not gene symbols from the fasta headers
All supplemental data including statistical analyses
The normalized count table for all experiments. Details on the column headers are available in a separate file, experimental design.txt
A key to column headers in the normalized count table.