Data from: The many dimensions of diet breadth: phytochemical, genetic, behavioral, and physiological perspectives on the interaction between a native herbivore and an exotic host
Harrison, Joshua G., University of Nevada Reno
Gompert, Zachariah, Utah State University
Fordyce, James A., University of Tennessee at Knoxville
Buerkle, C. Alex, University of Wyoming
Grinstead, Rachel, University of Nevada Reno
Jahner, Joshua P., University of Nevada Reno
Mikel, Scott, University of Nevada Reno
Nice, Christopher C., Texas State University
Santamaria, Aldrin, University of Nevada Reno
Forister, Matthew L., University of Nevada Reno
Published Apr 20, 2016 on Dryad.
Cite this dataset
Harrison, Joshua G. et al. (2016). Data from: The many dimensions of diet breadth: phytochemical, genetic, behavioral, and physiological perspectives on the interaction between a native herbivore and an exotic host [Dataset]. Dryad. https://doi.org/10.5061/dryad.g71c0
From the perspective of an herbivorous insect, conspecific host plants are not identical, and intraspecific variation in host nutritional quality or defensive capacity might mediate spatially variable outcomes in plant-insect interactions. Here we explore this possibility in the context of an ongoing host breadth expansion of a native butterfly (the Melissa blue, Lycaeides melissa) onto an exotic host plant (alfalfa, Medicago sativa). We examine variation among seven alfalfa populations that differed in terms of colonization by L. melissa; specifically, we examined variation in phytochemistry, foliar protein, and plant population genetic structure, as well as responses of caterpillars and adult butterflies to foliage from the same populations. Regional patterns of alfalfa colonization by L. melissa were well predicted by phytochemical variation, and colonized patches of alfalfa showed a similar level of inter-individual phytochemical diversity. However, phytochemical variation was a poor predictor of larval performance, despite the fact that survival and weight gain differed dramatically among caterpillars reared on plants from different alfalfa populations. Moreover, we observed a mismatch between alfalfa supporting the best larval performance and alfalfa favored by ovipositing females. Thus, the axes of plant variation that mediate interactions with L. melissa depend upon herbivore life history stage, which raises important issues for our understanding of adaptation to novel resources by an organism with a complex life history.
Alfalfa chemistry data
Phytochemical data for alfalfa (Medicago sativa) samples. This csv file includes sample meta-data, and chemical measurements for 49 anonymous chemicals.
Alfalfa protein data
Protein data for alfalfa (Medicago sativa) samples. This csv file includes sample meta-data and protein absorbency measurements.
Larval performance data
Lycaeides melissa larval performance data. This spreadsheet includes plant population, survival and mass data for individual caterpillars reared on alfalfa from different sites.
Oviposition preference data
This compressed directory gives the oviposition preference data in terms of the number of eggs laid on individual plants by different females. Four files are included with different butterfly (SLA vs. VUH) and alfalfa populations.
Draft alfalfa genome assembly
FASTA file contains the draft alfalfa genome described in the paper, which is organized in scaffolds. The assembly was generated with ALLPATHS-LG. The shell script used to run the assembly is included as a ReadMe file.
Alfalfa genetic data
Filtered genetic data for population samples of alfalfa. The file is in vcf format and was produced using GATK's UnifiedGenotyper with ploidy set to 4 (i.e., assuming tertraploids). Only variants with a mean of 2X coverage were retained in this file. IDs provide population information as described in the paper.