Nanopore sequencing genomic DNA from Stentor pyriformis and its endosymbiont, Chlorella variabilis
Data files
Jan 28, 2025 version files 12.76 GB
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chlorella.dna.gridion_hac.fastq.gz
2.48 GB
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chlorella.rna.gridion_hac.fastq.gz
2.62 GB
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README.md
1.17 KB
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stentor_DNA.guppy4.2.2_hac.fastq.gz
3.35 GB
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stentor.RNA.guppy4.2.2_hac.fastq.gz
4.31 GB
Abstract
Stentor pyriformis are giant, unicellular ciliates that harbor an algal endosymbiont that we have identified as Chlorella variabilis. These organisms are a new model system for mixotrophy in ciliates, which has primarily been studied in Paramecium bursaria and its endosymbiont, also Chlorella variabilis. Understanding the similarities and differences between the genomes in these endosymbioses will ultimately aid our understanding of the evolution of mixotrophy, endosymbiosis, and organelle fixation.
README: Nanopore sequencing genomic DNA from Stentor pyriformis and its endosymbiont, Chlorella variabilis
https://doi.org/10.5061/dryad.g79cnp612
Description of the data and file structure
Stentor pyriformis are an emerging model for studying mixotrophy in ciliates, which is primarily studied in Paramecium bursaria. Sequencing and annotating the genomes of both S. pyriformis, and its endosymbiont, which we identified as Chlorella variabilis (see related work), is important for our understanding of the relationship between these two species.
Files
File: stentor_DNA.guppy4.2.2_hac.fastq.gz
Description: Nanopore DNA sequencing data from Stentor pyriformis
File: stentor.RNA.guppy4.2.2_hac.fastq.gz
Description: Nanopore RNA sequencing data from Stentor pyriformis
File: chlorella.rna.gridion_hac.fastq.gz
Description: Nanopore RNA sequencing data from Chlorella variabilis (endosymbiont of S. pyriformis)
File: chlorella.dna.gridion_hac.fastq.gz
Description: Nanopore DNA sequencing data from Chlorella variabilis (endosymbiont of S. pyriformis)
Methods
Samples were sequenced with an Oxford Nanopore MinION or GridION. Draft assemblies and annotation information can be found at https://stentor.ciliate.org/, and are described in the associated preprint.