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Pollen defenses negatively impact foraging and fitness in a generalist bee (Bombus impatiens: Apidae)

Citation

Brochu, Kristen (2020), Pollen defenses negatively impact foraging and fitness in a generalist bee (Bombus impatiens: Apidae), Dryad, Dataset, https://doi.org/10.5061/dryad.gb5mkkwks

Abstract

Plants may benefit from limiting the community of generalist floral visitors if the species that remain are more effective pollinators and less effective pollenivores. Plants can reduce access to pollen through altered floral cues or morphological structures, but can also reduce consumption through direct pollen defenses. We observed that Eucera (Peponapis) pruinosa, a specialist bee on Cucurbita plants, collected pure loads of pollen while generalist honey bees and bumble bees collected negligible amounts of cucurbit pollen, even though all groups of bees visited these flowers. Cucurbit flowers have no morphological adaptations to limit pollen collection by bees, thus we assessed their potential for physical, nutritional, and chemical pollen traits that might act as defenses to limit pollen loss to generalist pollinators. Bumble bee (Bombus impatiens) microcolonies experienced reduced pollen consumption, mortality, and reproduction as well as increased stress responses when exposed to nutritional and mechanical pollen defenses. These bees also experienced physiological effects of these defenses in the form of hindgut expansion and gut melanization. Chemical defenses alone increased the area of gut melanization in larger bees and induced possible compensatory feeding. Together, these results suggest that generalist bumble bees avoid collecting cucurbit pollen due to the physiological costs of physical and chemical pollen defenses.

Methods

We completed a field experiment to collect pollen counts from bees found in Cucurbit fields. We also completed a laboratory microcolony assay to assess how Bombus impatiens is impacted by feeding on a cucurbit pollen diet. Please see the article for further information.

Usage Notes

Please see the PollenDefensesDataREADME.txt file for data details.

Funding

National Institute of Food and Agriculture Hatch Grant, Award: NYC-139426

Canadian Natural Sciences and Engineering Research Council Postgraduate Scholarship - Doctoral, Award: PGSD3-425709-2012

Griswold Fund of the Cornell University Entomology Department