Additive and mostly adaptive plastic responses of gene expression to multiple stress in Tribolium castaneum
Koch, Eva; Guillaume, Frédéric (2020), Additive and mostly adaptive plastic responses of gene expression to multiple stress in Tribolium castaneum, Dryad, Dataset, https://doi.org/10.5061/dryad.gf1vhhmkn
We used a T. castaneum strain (Cro1) which was collected from a wild population in 2010 and adapted to standard control conditions (CTL: 33 °C, 70% relative humidity (r.h.)) since then. To assess the fitness changes caused by stressful environmental conditions, we exposed the beetles to a drought, a heat, and a combined heat-drought treatment (conditions: Dry (=D) : 33°C, 30% r.h.; Hot (=H): 37 °C, 70 % r.h; Hot-Dry (=HD): 37°C, 30% r.h.). Parents of the experimental beetles were reared in control conditions and beetles used for this fitness assay were transferred to treatments at the egg stage. Males and females were separated at the pupal stage. We mated each virgin female with one unrelated male from the same condition in a 15 mL tube with 1 g medium (organic wheat flour mixed with 10% organic baker's yeast). The male was removed after 24 h. Females were removed from the tubes after one week of egg laying, and 9 g medium was added to provide food for the developing offspring. After five weeks the number of offspring was counted. At this time, all offspring had reached the adult stage. Some females did not produce any offspring. Data was collected in 2015.
First column: female ID = female that produced offspring; second column: male ID = mating partner of female, father of offspring; third column: number of adult offspring; fourth column: condition in the climate chamber: CTL=control, D=dry, H=hot, HD=hot-dry
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, Award: PP00P3_1144846
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, Award: PP00P3_176965