Data from: Hybridization has localized effect on genetic variation in closely related pine species
Data files
Aug 22, 2024 version files 436.82 KB
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mtDNA.xlsx
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nSSR.xlsx
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README.md
Abstract
Background: Hybridization is a known phenomenon in nature but its genetic impact on populations of parental species remains less understood. We investigated the evolutionary consequences of the interspecific gene flow in several contact zones of closely related pine species. Using a set of genetic markers from both nuclear and organellar genomes, we analyzed a large panel of reference allopatric populations of parental taxa (96 stands, 2601 individuals).
Results: We observed reduced genetic diversity in maternally transmitted mitochondrial genomes of pure pine species and hybrids from contact zones compared to reference allopatric populations. The distribution of mtDNA haplotypes followed geographic rather than species boundaries. Additionally, no new haplotypes emerged in the contact zones, instead, these zones contained the most common local variants. However, species diverged significantly at nuclear genomes, and populations in contact zones exhibited similar or higher genetic diversity compared to the reference stands. There were no signs of admixture in any allopatric population, while clear admixture was evident in the contact zones, indicating that hybridization has a localized effect on the genetic variation of the analyzed pine species.
Conclusions: Our results suggest that hybrid zones act as sinks rather than melting pots of genetic diversity. Hybridization influences sympatric populations but is confined to contact zones. The spectrum of parental species ancestry in hybrids reflects the old evolutionary history of the sympatric populations. These findings also imply that introgression may play a crucial role in the adaptation of hybrids to specific environments.
README: Data from: Hybridization has localized effect on genetic variation in closely related pine species
https://doi.org/10.5061/dryad.gf1vhhmxn
Description of the data and file structure
Genetic data is divided in two files - one for nuclear and one for mitochondrial data. Nuclear data is a set of 14 polymorphic microsatellites designed for pines. Mitochondrial data is a set of 13 markers (Single Nucleotide Polymorphisms (SNPs) and an indel marker coded as a SNP) generated using a SNaPshot approach. The first two rows of both files contain population and marker information generated by GenAlEx.
Files and variables
File: mtDNA.xlsx
Description: The mtDNA genotypes of 2601 pine individuals scored at 13 polymorphic mtDNA regions. The column sample contains individual ID, and pop population ID.
File: nSSR.xlsx
Description: The table contains genotype data scored from 2073 pine individuals across 73 populations. Rounded allele values are provided for 14 SSR loci. The first two columns are for individual ID and population ID, respectively, and the remaining columns are for the 14 SSR loci (2 columns per locus). The table includes a header with column names.
Code/software
Both files can be open using Microsoft Excel and are prepared in GenAlEx 6.5 format.