Data from: Dietary specialization in mutualistic acacia-ants affects relative abundance but not identity of host-associated bacteria
Rubin, Benjamin E. R.; Kautz, Stefanie; Wray, Brian D.; Moreau, Corrie S. (2018), Data from: Dietary specialization in mutualistic acacia-ants affects relative abundance but not identity of host-associated bacteria, Dryad, Dataset, https://doi.org/10.5061/dryad.gg6n17s
Acacia-ant mutualists in the genus Pseudomyrmex nest obligately in acacia plants and, as we show through stable isotope analysis, feed at a remarkably low trophic level. Insects with diets such as these sometimes depend on bacterial symbionts for nutritional enrichment. We, therefore, examine the bacterial communities associated with acacia-ants in order to determine whether they host bacterial partners likely to contribute to their nutrition. Despite large differences in trophic position, acacia-ants and related species with generalized diets do not host distinct bacterial taxa. However, we find that a small number of previously undescribed bacterial taxa do differ in relative abundance between acacia-ants and generalists, including several Acetobacteraceae and Nocardiaceae lineages related to common insect-associates. Comparisons with an herbivorous generalist, a parasite that feeds on acacias, and a mutualistic species with a generalized diet show that trophic level is likely responsible for these small differences in bacterial community structure. While we did not experimentally test for a nutritional benefit to hosts of these bacterial lineages, metagenomic analysis reveals a Bartonella relative with an intact nitrogen-recycling pathway widespread across Pseudomyrmex mutualists and generalists. This taxon may be contributing to nitrogen enrichment of its ant hosts through urease activity and, concordant with an obligately host-associated lifestyle, appears to be experiencing genome-wide relaxed selection. The lack of distinctiveness in bacterial communities across trophic level in this group of ants shows a remarkable ability to adjust to varied diets, possibly with assistance from these diverse ant-specific bacterial lineages.
National Science Foundation, Award: DEB-1311417, DEB-1050243, DEB-1442316