Experimental peritoneal dialysis in Sprague-Dawley rats using conventional or low sodium dialysis fluids
Abstract
During peritoneal dialysis, sodium is transferred in a paracellular fashion via inter-endothelial nanochannels from the blood plasma water to the dialysate and, initially, dialysate sodium concentrations are also diluted by free water transport across endothelial aquaporin-1 channels. These experiments were performed to evaluate the relative contributions of free water transport, convective- and diffusive transfer on dialysate sodium. Based on our findings, we devised a simple method to quantify ultrafiltration by analyzing the initial decline in dialysate sodium concentration, commonly referred to as the "sodium dip."
README: Experimental peritoneal dialysis in Sprague-Dawley rats using conventional or low sodium dialysis fluids
GENERAL INFORMATION
Title of Dataset: Experimental Peritoneal Dialysis in Sprague Dawley Rats: Low Sodium versus Conventional Dialysis Fluids
Author Information
A. Principal Investigator Contact Information
Name: Carl berg
Institution: Lund University, Medical Faculty
Address: Box 117, SE-221 00
Email: carl.oberg@med.lu.seDate of data collection March 2022 to April 2022
Geographic location of data collection: Lund, Sweden
Information about funding sources that supported the collection of the data: Funding by Lund University Medical Faculty Foundation grant YF 2020-YF0056, and The Inga-Britt and Arne Lundbergs Research Foundation (all to C.M. berg).
SHARING/ACCESS INFORMATION
Licenses/restrictions placed on the data: -
Links to publications that cite or use the data: -
Links to other publicly accessible locations of the data: -
Links/relationships to ancillary data sets: -
Was data derived from another source? No
Recommended citation for this dataset: -
DATA & FILE OVERVIEW
File List: lowna.csv
Relationship between files, if important: -
Additional related data collected that was not included in the current data package: -
Are there multiple versions of the dataset? NO
METHODOLOGICAL INFORMATION
Description of methods used for collection/generation of data: -
Methods for processing the data: -
Instrument- or software-specific information needed to interpret the data: -
Standards and calibration information, if appropriate: -
Environmental/experimental conditions: -
Describe any quality-assurance procedures performed on the data: -
People involved with sample collection, processing, analysis and/or submission: -
DATA-SPECIFIC INFORMATION FOR: lowna.csv
Number of variables: 148
Number of cases/rows: 24
Variable List:
animal, "unique number assigned to each animal"
treatment, "Dialysis fluid used, e.g. LowNa 1.5% represents low sodium and 1.5% glucose strength"
weight, "Body weight in grams before dialysis"
date, "Date of the experiment YYYY-MM-DD"
vin, "Peritoneal dialysis fluid volume instilled in milliliters"
vout, "Peritoneal dialysis fluid volume extracted in milliliters (see manuscript for details)"
wo_in, "Wash-out fluid instilled (same type of fluid as used for dialysis) in milliliters"
wo_out, "Wash-out fluid extracted in milliliters"
wo_alb, "Wash-out fluid 125-I activity in Bq/mL"
p_na_before, "Plasma sodium concentration before dialysis in mmol/L"
p_k_before, "Plasma potassium concentration before dialysis in mmol/L"
p_cl_before, "Plasma chloride concentration before dialysis in mmol/L"
p_ica_before, "Plasma ionized calcium concentration before dialysis in mmol/L"
p_tco2_before, "Plasma total carbon dioxide concentration before dialysis in mmol/L"
p_glu_before, "Plasma glucose concentration before dialysis in mmol/L"
p_urea_before, "Plasma urea concentration before dialysis in mmol/L"
p_crea_before, "Plasma creatinine concentration before dialysis in mmol/L"
map_before, "Mean arterial pressure before dialysis in mmol/L"
hr_before, "Heart rate before dialysis in mmol/L"
p_na_after, "Plasma sodium concentration after dialysis in mmol/L"
p_k_after, "Plasma potassium concentration after dialysis in mmol/L"
p_cl_after, "Plasma chloride concentration after dialysis in mmol/L"
p_ica_after, "Plasma ionized calcium concentration after dialysis in mmol/L"
p_tco2_after, "Plasma total carbon dioxide concentration before dialysis in mmol/L"
p_glu_after, "Plasma glucose concentration after dialysis in mmol/L"
p_urea_after, "Plasma urea concentration after dialysis in mmol/L"
p_crea_after, "Plasma creatinine concentration after dialysis in mmol/L"
map_after, "Mean arterial pressure before dialysis in mmol/L"
hr_after, "Heart rate before dialysis in mmol/L"
n_p_samples, "Number of blood samples obtained during dialysis"
pt1, "Time point for blood sample #1 in minutes post start of dialysis"
pt2, "Time point for blood sample #2 in minutes post start of dialysis"
pt3, "Time point for blood sample #3 in minutes post start of dialysis"
pt4, "Time point for blood sample #4 in minutes post start of dialysis"
pt5, "Time point for blood sample #5 in minutes post start of dialysis"
pt6, "Time point for blood sample #6 in minutes post start of dialysis"
p_hct_1, "Hematocrit for blood sample #1"
p_hct_2, "Hematocrit for blood sample #2"
p_hct_3, "Hematocrit for blood sample #3"
p_hct_4, "Hematocrit for blood sample #4"
p_hct_5, "Hematocrit for blood sample #5"
p_hct_6, "Hematocrit for blood sample #6"
p_edta_1, "Plasma 51Cr activity (blood sample #1) in Bq/mL"
p_edta_2, "Plasma 51Cr activity (blood sample #2) in Bq/mL"
p_edta_3, "Plasma 51Cr activity (blood sample #3) in Bq/mL"
p_edta_4, "Plasma 51Cr activity (blood sample #4) in Bq/mL"
p_edta_5, "Plasma 51Cr activity (blood sample #5) in Bq/mL"
p_edta_6, "Plasma 51Cr activity (blood sample #6) in Bq/mL"
p_alb_1, "Plasma 125I activity (blood sample #1) in Bq/mL"
p_alb_2, "Plasma 125I activity (blood sample #2) in Bq/mL"
p_alb_3, "Plasma 125I activity (blood sample #3) in Bq/mL"
p_alb_4, "Plasma 125I activity (blood sample #4) in Bq/mL"
p_alb_5, "Plasma 125I activity (blood sample #5) in Bq/mL"
p_alb_6, "Plasma 125I activity (blood sample #6) in Bq/mL"
n_d_samples, "Number of dialysate samples obtained during dialysis"
d_sv_1, "Sampling volume for dialysate sample #1 in milliliters"
d_sv_2, "Sampling volume for dialysate sample #2 in milliliters"
d_sv_3, "Sampling volume for dialysate sample #3 in milliliters"
d_sv_4, "Sampling volume for dialysate sample #4 in milliliters"
d_sv_5, "Sampling volume for dialysate sample #5 in milliliters"
d_sv_6, "Sampling volume for dialysate sample #6 in milliliters"
d_sv_7, "Sampling volume for dialysate sample #7 in milliliters"
d_edta_0, "51Cr activity in fresh dialysis fluid (before instillation) in Bq/mL"
d_edta_1, "51Cr activity in dialysate sample #1 in Bq/mL"
d_edta_2, "51Cr activity in dialysate sample #2 in Bq/mL"
d_edta_3, "51Cr activity in dialysate sample #3 in Bq/mL"
d_edta_4, "51Cr activity in dialysate sample #4 in Bq/mL"
d_edta_5, "51Cr activity in dialysate sample #5 in Bq/mL"
d_edta_6, "51Cr activity in dialysate sample #6 in Bq/mL"
d_edta_7, "51Cr activity in dialysate sample #7 in Bq/mL"
d_alb_0, "125I activity in fresh dialysis fluid (before instillation) in Bq/mL"
d_alb_1, "125I activity in dialysate sample #1 in Bq/mL"
d_alb_2, "125I activity in dialysate sample #2 in Bq/mL"
d_alb_3, "125I activity in dialysate sample #3 in Bq/mL"
d_alb_4, "125I activity in dialysate sample #4 in Bq/mL"
d_alb_5, "125I activity in dialysate sample #5 in Bq/mL"
d_alb_6, "125I activity in dialysate sample #6 in Bq/mL"
d_alb_7, "125I activity in dialysate sample #7 in Bq/mL"
d_glu_0, "Measured glucose concentration in fresh dialysis fluid (before instillation) in mmol/L"
d_glu_1, "Measured glucose concentration in dialysate sample #1 in mmol/L"
d_glu_2, "Measured glucose concentration in dialysate sample #2 in mmol/L"
d_glu_3, "Measured glucose concentration in dialysate sample #3 in mmol/L"
d_glu_4, "Measured glucose concentration in dialysate sample #4 in mmol/L"
d_glu_5, "Measured glucose concentration in dialysate sample #5 in mmol/L"
d_glu_6, "Measured glucose concentration in dialysate sample #6 in mmol/L"
d_glu_7, "Measured glucose concentration in dialysate sample #7 in mmol/L"
d_na_0, "Measured sodium concentration in fresh dialysis fluid (before instillation) in mmol/L"
d_na_1, "Measured glucose concentration in dialysate sample #1 in mmol/L"
d_na_2, "Measured glucose concentration in dialysate sample #2 in mmol/L"
d_na_3, "Measured glucose concentration in dialysate sample #3 in mmol/L"
d_na_4, "Measured glucose concentration in dialysate sample #4 in mmol/L"
d_na_5, "Measured glucose concentration in dialysate sample #5 in mmol/L"
d_na_6, "Measured glucose concentration in dialysate sample #6 in mmol/L"
d_na_7, "Measured glucose concentration in dialysate sample #7 in mmol/L"
d_k_0, "Measured potassium concentration in fresh dialysis fluid (before instillation) in mmol/L"
d_k_1, "Measured potassium concentration in dialysate sample #1 in mmol/L"
d_k_2, "Measured potassium concentration in dialysate sample #2 in mmol/L"
d_k_3, "Measured potassium concentration in dialysate sample #3 in mmol/L"
d_k_4, "Measured potassium concentration in dialysate sample #4 in mmol/L"
d_k_5, "Measured potassium concentration in dialysate sample #5 in mmol/L"
d_k_6, "Measured potassium concentration in dialysate sample #6 in mmol/L"
d_k_7, "Measured potassium concentration in dialysate sample #7 in mmol/L"
d_cl_0, "Measured chloride concentration in fresh dialysis fluid (before instillation) in mmol/L"
d_cl_1, "Measured chloride concentration in dialysate sample #1 in mmol/L"
d_cl_2, "Measured chloride concentration in dialysate sample #2 in mmol/L"
d_cl_3, "Measured chloride concentration in dialysate sample #3 in mmol/L"
d_cl_4, "Measured chloride concentration in dialysate sample #4 in mmol/L"
d_cl_5, "Measured chloride concentration in dialysate sample #5 in mmol/L"
d_cl_6, "Measured chloride concentration in dialysate sample #6 in mmol/L"
d_cl_7, "Measured chloride concentration in dialysate sample #7 in mmol/L"
d_ica_0, "Measured ionized calcium concentration in fresh dialysis fluid (before instillation) in mmol/L"
d_ica_1, "Measured ionized calcium concentration in dialysate sample #1 in mmol/L"
d_ica_2, "Measured ionized calcium concentration in dialysate sample #2 in mmol/L"
d_ica_3, "Measured ionized calcium concentration in dialysate sample #3 in mmol/L"
d_ica_4, "Measured ionized calcium concentration in dialysate sample #4 in mmol/L"
d_ica_5, "Measured ionized calcium concentration in dialysate sample #5 in mmol/L"
d_ica_6, "Measured ionized calcium concentration in dialysate sample #6 in mmol/L"
d_ica_7, "Measured ionized calcium concentration in dialysate sample #7 in mmol/L"
d_tco2_0, "Measured total carbon dioxide concentration in fresh dialysis fluid (before instillation) in mmol/L"
d_tco2_1, "Measured total carbon dioxide concentration in dialysate sample #1 in mmol/L"
d_tco2_2, "Measured total carbon dioxide concentration in dialysate sample #1 in mmol/L"
d_tco2_3, "Measured total carbon dioxide concentration in dialysate sample #1 in mmol/L"
d_tco2_4, "Measured total carbon dioxide concentration in dialysate sample #1 in mmol/L"
d_tco2_5, "Measured total carbon dioxide concentration in dialysate sample #1 in mmol/L"
d_tco2_6, "Measured total carbon dioxide concentration in dialysate sample #1 in mmol/L"
d_tco2_7, "Measured total carbon dioxide concentration in dialysate sample #1 in mmol/L"
d_urea_1, "Measured urea concentration in dialysate sample #1 in mmol/L"
d_urea_2, "Measured urea concentration in dialysate sample #2 in mmol/L"
d_urea_3, "Measured urea concentration in dialysate sample #3 in mmol/L"
d_urea_4, "Measured urea concentration in dialysate sample #4 in mmol/L"
d_urea_5, "Measured urea concentration in dialysate sample #5 in mmol/L"
d_urea_6, "Measured urea concentration in dialysate sample #6 in mmol/L"
d_urea_7, "Measured urea concentration in dialysate sample #7 in mmol/L"
d_crea_0, "Measured creatinine concentration in fresh dialysis fluid (before instillation) in mol/L"
d_crea_1, "Measured creatinine concentration in dialysate sample #2 in mol/L"
d_crea_2, "Measured creatinine concentration in dialysate sample #2 in mol/L"
d_crea_3, "Measured creatinine concentration in dialysate sample #2 in mol/L"
d_crea_4, "Measured creatinine concentration in dialysate sample #2 in mol/L"
d_crea_5, "Measured creatinine concentration in dialysate sample #2 in mol/L"
d_crea_6, "Measured creatinine concentration in dialysate sample #2 in mol/L"
d_crea_7, "Measured creatinine concentration in dialysate sample #2 in mol/L"
dt1, "Time point for dialysate sample #1 in minutes post start of dialysis"
dt2, "Time point for dialysate sample #2 in minutes post start of dialysis"
dt3, "Time point for dialysate sample #3 in minutes post start of dialysis"
dt4, "Time point for dialysate sample #4 in minutes post start of dialysis"
dt5, "Time point for dialysate sample #5 in minutes post start of dialysis"
dt6, "Time point for dialysate sample #6 in minutes post start of dialysis"
dt7, "Time point for dialysate sample #7 in minutes post start of dialysis"
Missing data codes: NA
Specialized formats or other abbreviations used: -
Methods
Experimental peritoneal dialysis was performed in healthy anesthetized 9 weeks old Sprague-Dawley rats using either 20 mL conventional (134 mmol/L) or low-sodium (125 mmol/L) peritoneal dialysis fluids utilizing a dwell time of 120 minutes. Blood plasma was analysed before and after dialysis to measure Na+, K+, Cl-. Ca++, glucose, urea, creatinine, and total CO2. 125I-albumin and 51Cr-EDTA were added to the dialysate to estimate intra-peritoneal volume and to assess small solute diffusion capacity, respectively. Samples (20 μL) of dialysate and blood were collected at multiple time-points during the dialysis session and analyzed on a gamma counter. All data in this dataset are unprocessed.