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Dryad

Construction and characterization of a genome-scale ordered mutant collection of Bacteroides thetaiotaomicron

Cite this dataset

Shiver, Anthony et al. (2022). Construction and characterization of a genome-scale ordered mutant collection of Bacteroides thetaiotaomicron [Dataset]. Dryad. https://doi.org/10.5061/dryad.gqnk98ss9

Abstract

Ordered transposon-insertion collections, in which specific transposon-insertion mutants are stored as monocultures in a genome-scale collection, represent a promising tool for genetic dissection of human gut microbiota members. However, publicly available collections are scarce and the construction methodology remains in the early stages of development. This dataset contains the raw data associated with the statistics and figures reported in an accompanying paper which describes the assembly of a genome-scale ordered collection of transposon-insertion mutants in the model gut anaerobe Bacteroides thetaiotaomicron VPI-5482 that we created as a resource for the research community.

Funding

National Institute of General Medical Sciences, Award: RM1: GM135102

National Institute of Allergy and Infectious Diseases, Award: R01: AI147023