Data from: Trpc2 Pseudogenization dynamics in bats reveal ancestral vomeronasal signaling, then pervasive loss
Yohe, Laurel R., Stony Brook University
Abubakar, Ramatu, Stony Brook University
Giordano, Christina, Stony Brook University
Dumont, Elizabeth, University of Massachusetts Amherst
Sears, Karen, Stony Brook University
Rossiter, Stephen J., Queen Mary University of London
Davalos, Liliana M., Stony Brook University
Sears, Karen E., University of Illinois System
Published Jan 09, 2017 on Dryad.
Cite this dataset
Yohe, Laurel R. et al. (2017). Data from: Trpc2 Pseudogenization dynamics in bats reveal ancestral vomeronasal signaling, then pervasive loss [Dataset]. Dryad. https://doi.org/10.5061/dryad.gr1pk
Comparative methods are often used to infer loss or gain of complex phenotypes, but few studies take advantage of genes tightly linked with complex traits to test for shifts in the strength of selection. In mammals vomerolfaction detects chemical cues mediating many social and reproductive behaviors and is highly conserved, but all bats exhibit degraded vomeronasal structures with the exception of two families (Phyllostomidae and Miniopteridae). These families either regained vomerolfaction after ancestral loss, or there were many independent losses after diversification from an ancestor with functional vomerolfaction. In this study, we use the Transient receptor potential cation channel 2 (Trpc2) as a molecular marker for testing the evolutionary mechanisms of loss and gain of the mammalian vomeronasal system. We sequenced Trpc2 exon 2 in over 100 bat species across 17 of 20 chiropteran families. Most families showed independent pseudogenizing mutations in Trpc2, but the reading frame was highly conserved in phyllostomids and miniopterids. Phylogeny-based simulations suggest loss of function occurred after bat families diverged, and purifying selection in two families has persisted since bats shared a common ancestor. As most bats still display pheromone-mediated behavior, they might detect pheromones through the main olfactory system without using the Trpc2 signaling mechanism.
This file contains controls files and output for gene tree inference (Garli), control files for evolver simulations (evolver), control files, input files, and output for both PAML (PAML_codeml) and RELAX analyses (RELAX), and R code to make Figures 4 and 5.
National Science Foundation, Award: DEB-1442142, GRFP