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Data from: Clathrin-independent endocytic retrieval of SV proteins mediated by the clathrin adaptor AP-2 at mammalian central synapses

Citation

Takamori, Shigeo; Haucke, Volker; Lopez-Hernandez, Tania; Takenaka, Koh-ichiro (2022), Data from: Clathrin-independent endocytic retrieval of SV proteins mediated by the clathrin adaptor AP-2 at mammalian central synapses, Dryad, Dataset, https://doi.org/10.5061/dryad.gxd2547mc

Abstract

Neurotransmission is based on the exocytic fusion of synaptic vesicles (SVs) followed by endocytic membrane retrieval and the reformation of SVs. Conflicting models have been proposed regarding the mechanisms of SV endocytosis, most notably clathrin/ AP-2-mediated endocytosis and clathrin-independent ultrafast endocytosis. Partitioning between these pathways has been suggested to be controlled by temperature and stimulus paradigm. We report on the comprehensive survey of six major SV proteins to show that SV endocytosis in mouse hippocampal neurons at physiological temperature occurs independent of clathrin while the endocytic retrieval of a subset of SV proteins including the vesicular transporters for glutamate and GABA depend on sorting by the clathrin adaptor AP-2. Our findings highlight a clathrin-independent role of the clathrin adaptor AP-2 in the endocytic retrieval of select SV cargos from the presynaptic cell surface and suggest a revised model for the endocytosis of SV membranes at mammalian central synapses.

Funding

Japan Society for the Promotion of Science, Award: 19H03330

Japan Society for the Promotion of Science, Award: JPJSCCA20170008

Deutsche Forschungsgemeinschaft, Award: SFB958/TP A01

Takeda Science Foundation

Deutsche Forschungsgemeinschaft, Award: HA 2686/13-1

The Watanabe Foundation Scholarship grant, Award: WS2021-013