Data from: Can females differentially allocate resources to offspring sired by different males?
Data files
Jun 06, 2024 version files 94.82 KB
-
HFAIAnalysis.R
3.62 KB
-
HFAIMaster2022.csv
88.76 KB
-
README.md
2.44 KB
Abstract
The viviparity-driven conflict hypothesis postulates that the evolution of matrotrophy (postfertilization maternal provisioning) will result in a shift from a pre- to postcopulatory mate choice and thus accelerate the evolution of postcopulatory reproductive isolation. Here, we perform artificial insemination experiments on Heterandria formosa, a matrotrophic poeciliid fish, to probe for evidence of postcopulatory female choice. We established laboratory populations from Wacissa River (WR) and Lake Jackson (LJ). The WR females normally produce larger offspring than the LJ females. We artificially inseminated females with sperm from each population or from both populations simultaneously. When LJ females were inseminated with sperm from WR and LJ males, they allocated fewer resources to WR-sired offspring than when they were inseminated with WR sperm alone. The LJ females carrying developing offspring sired by males from different populations were thus able to discriminate against non-resident males when allocating resources to developing young. The WR females, which normally produce larger offspring than LJ females, did not discriminate among males from different localities. These findings provide insights into the ability of females from one population to exercise a form of postcopulatory mate selection.
README: Can females differentially allocate resources to offspring sired by different males?
https://doi.org/10.5061/dryad.h18931ztf
We inseminated female Heterandria formosa with spermatozeugmata from either two or four males, comprising four treatments: (A) female × 2 WR males; (B) female × 2 LJ males; (C) female × 1 WR male + 1 LJ male; and (D) female × 2 WR males + 2 LJ males. These treatments were later combined into ‘Single’ male population and ‘Mixed’ male population insemination treatments. These data include offspring weights and genotypes from fish born in this experiment. The data associated with each offspring included: days since insemination, number of broods per female, and number of offspring per brood. Any offspring born within a 48 h interval were counted towards a single brood, because there is typically a 3- to 5-day interval between broods, and two broods born within 48 h is highly unlikely.
Description of the data and file structure
Here is a key to the .csv file (mg = milligrams, g=grams):
ofid = Offspring identification number
offscount = the number of offspring represented in the row (for summing purposes)
ETOHWeight = ethanol weight in mg
wet_wt = wet weight in g
wet_wt_mg = wet weight in mg
dam_id = the dam's identification number
dam_pop = the originating population (Wacissa River/WR or Lake Jackson/LJ) of the dam
Treatment = (A) female × 2 WR males; (B) female × 2 LJ males; (C) female × 1 WR male + 1 LJ male; and (D) female × 2 WR males + 2 LJ males
MomWTI = dam weight at the beginning of the experiment
brood = the brood from which the offspring originated
sire_id = the sire's identification number as determined by genotyping
treat_type = S for single with two males from the same population or M for Mixed insemination with one male from each population
sire_pop = the originating population (Wacissa River/WR or Lake Jackson/LJ) of the sire determined by genotyping
SireTLI = total length for the sire as determined by genotyping
Note: cells containing NA mean there is no relevant or recorded data available for that cell.
Code/Software
An annotated R script is included. At the time of publication, version R4.2.2 was used. All loaded packages are included in the script, as well as here:
tidyverse
lme4
car
emmeans
plotrix
modelbased
ggpubr
Methods
We inseminated female Heterandria formosa with spermatozeugmata from either two or four males, comprising four treatments: (A) female × 2 WR males; (B) female × 2 LJ males; (C) female × 1 WR male + 1 LJ male; and (D) female × 2 WR males + 2 LJ males. These treatments were later combined into ‘Single’ male population and ‘Mixed’ male population insemination treatments. These data include offspring weights and genotypes from fish born in this experiment. The data associated with each offspring included: days since insemination, number of broods per female, and number of offspring per brood. Any offspring born within a 48 h interval were counted towards a single brood, because there is typically a 3- to 5-day interval between broods, and two broods born within 48 h is highly unlikely.