1. Estimating feral cat population densities in urban environments can be difficult due to lack of public space and human interference. The purpose of this study was to use distance sampling in a citywide landscape to determine population size and areas of high abundance to inform trap-neuter-release management programs. 2. Line transect distance sampling was used to estimate density of the feral cat population in Windsor, Ontario from June to July 2014. Windsor has a human population of 217,188 and is about 146 km2 in size. Most transects were placed along local roads. 3. Density was estimated at about 13.3 (95% CI 9.7 – 18.1) cats per km2, and an estimated population size of 1858 cats (95% CI 1361 – 2537) with the highest relative density occurring in West and Central Windsor. 4. Urban wildlife managers could utilize these methods to monitor feral cat populations and evaluate the effectiveness of trap-neuter-release programs.