Data from: The adaptive potential of subtropical rainbowfish in the face of climate change: heritability and heritable plasticity for the expression of candidate genes
McCairns, Scott R. J., Flinders University
Smith, Steve, Flinders University, University of Veterinary Medicine
Sasaki, Minami, Flinders University
Bernatchez, Louis, Université Laval
Beheregaray, Luciano B., Flinders University
McCairns, R. J. Scott, Flinders University
Published Jan 19, 2016 on Dryad.
Cite this dataset
McCairns, Scott R. J. et al. (2016). Data from: The adaptive potential of subtropical rainbowfish in the face of climate change: heritability and heritable plasticity for the expression of candidate genes [Dataset]. Dryad. https://doi.org/10.5061/dryad.h40d8
Whilst adaptation and phenotypic plasticity might buffer species against habitat degradation associated with global climate change, few studies making such claims also possess the necessary and sufficient data to support them. Doing so requires demonstration of heritable variation in traits affecting fitness under new environmental conditions. We address this issue using an emerging aquatic system to study adaptation to climate change, the crimson-spotted rainbowfish (Melanotaenia duboulayi), a freshwater species from a region of eastern Australia projected to be affected by marked temperature increases. Captive born M. duboulayi of known pedigree were used to assess the long-term effects of contemporary and 2070-projected summer temperatures on the expression of genes previously identified in a climate change transcriptomics (RNA-Seq) experiment. Nearly all genes responded to increasing temperature. Significant additive genetic variance explained a moderate proportion of transcriptional variation for all genes. Most genes also showed broad-sense genetic variation in transcriptional plasticity. Additionally, molecular pathways of candidate genes co-occur with genes inferred to be under climate-mediated selection in wild M. duboulayi populations. Together, these results indicate the presence of existing variation in important physiological traits, and the potential for adaptive responses to a changing thermal environment.
mRNA transcription of candidate genes for thermal adaptation in the crimson-spotted rainbowfish.
Total mRNA was extracted from laboratory bred and reared individuals (F1); parents were wild-caught and originated from the Brisbane River. At 6 months of age, individuals from each family group were subjected to one of two thermal treatments for a duration of 80 days. Rearing tank (Tank), sex (Sex), thermal treatment group (Treat) individual identifier (animal), parents (DAM & SIRE) and full-sib family group (Fam) are provided for each individual. Immediately following thermal treatment, fish were euthanized and total mRNA extracted from liver tissues.
Transcript abundance was estimated via quantitative real-time PCR. LinRegPCR was used to convert raw CT values into estimates of initial/starting mRNA concentrations (N0). Initial mRNA concentrations were standardized relative to the geometric mean of two reference genes (geomean.REFS). Standardized values of candidate genes occupy the final 12 columns; gene symbols (column headings) are the same as those used in the accompanying article (McCairns et al. 2016).