Data from: Experimental evolution of an emerging plant virus in host genotypes that differ in their susceptibility to infection
Hillung, Julia, Universitat Politècnica de València
Cuevas, José M., Universitat Politècnica de València
Valverde, Sergi, Barcelona Biomedical Research Park
Elena, Santiago F., Santa Fe Institute
Published May 20, 2014 on Dryad.
Cite this dataset
Hillung, Julia; Cuevas, José M.; Valverde, Sergi; Elena, Santiago F. (2014). Data from: Experimental evolution of an emerging plant virus in host genotypes that differ in their susceptibility to infection [Dataset]. Dryad. https://doi.org/10.5061/dryad.h443d
This study evaluates the extent to which genetic differences among host individuals from the same species conditions the evolution of a plant RNA virus. We performed a three-fold replicated evolution experiment in which Tobacco etch potyvirus isolate At17b (TEV-At17b), adapted to Arabidopsis thaliana ecotype Ler-0, was serially passaged in five genetically heterogeneous ecotypes of A. thaliana. After 15 passages we found that evolved viruses improved their fitness, showed higher infectivity and stronger virulence in their local host ecotypes. The genome of evolved lineages was sequenced and putative adaptive mutations identified. Host-driven convergent mutations have been identified. Evidences supported selection for increased translational efficiency. Next, we sought for the specificity of virus adaptation by infecting all five ecotypes with all 15 evolved virus populations. We found that some ecotypes were more permissive to infection than others, and that some evolved virus isolates were more specialist/generalist than others. The bipartite network linking ecotypes with evolved viruses was significantly nested but not modular, suggesting that hard to infect ecotypes were infected by generalist viruses whereas easy to infect ecotypes were infected by all viruses, as predicted by a gene-for-gene model of infection.
Raw fitness data
File contains three sheets. First sheet contains the raw fitness data (technical replicates of RT-qPCR per biological replicate). Second sheet contains the significance test for each fitness value corrected using FDR for multiple comparisons. Third sheet contains a summary of fitness data. Columns labels are self-explicative.