Lactose operon sequences from: Nature reserves suppress evolutionary rescue from extinction
Data files
Dec 11, 2024 version files 304.96 KB
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Fragment(alignment).fas
21.66 KB
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Full_length(alignment).fas
282.30 KB
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README.md
992 B
Abstract
Escherichia coli K-12 GM4792 is incapable of utilizing lactose (lac-). However, when cultured in M9 medium containing both lactose and glucose as mixed carbon sources, lactose-utilizing reverse mutations (lac+) can be observed. We isolated and sequenced single clones of both lac- and lac+, and identified the molecular basis underlying the lactose utilization.
README: Lactose operon sequences
https://doi.org/10.5061/dryad.h70rxwdvb
Description of the data and file structure
Escherichia coli K-12 GM4792 is unable to utilize lactose (lac-). However, when grown in an M9 medium containing lactose and glucose as mixed carbon sources, we can observe reverse mutations that allow the bacteria to utilize lactose (lac+). We isolated and sequenced single clones of both lac- and lac+ strains, and the alignment of these sequences is presented.
Files and variables
File: Fragment(alignment).fas
Description: Fragmented sequences of lactose operon
File: Full_length(alignment).fas
Description: Full length sequence of lactose operon
Code/software
Any gene sequence alignment sofware such as BioEdit and DNAman can be used to view the data.
Access information
Other publicly accessible locations of the data:
- no
Data was derived from the following sources:
- no
Methods
Lactose operon sequences from single clones that are incapable of utilizing lactose (lac-) and those with lactose-utilizing reverse mutations (lac+) were obtained by PCR and sequencing, and these sequences were subsequently aligned.