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Lactose operon sequences from: Nature reserves suppress evolutionary rescue from extinction

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Dec 11, 2024 version files 304.96 KB

Abstract

Escherichia coli K-12 GM4792 is incapable of utilizing lactose (lac-). However, when cultured in M9 medium containing both lactose and glucose as mixed carbon sources, lactose-utilizing reverse mutations (lac+) can be observed. We isolated and sequenced single clones of both lac- and lac+, and identified the molecular basis underlying the lactose utilization.