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FT TFL1 bimolecular luciferase complementation assays

Citation

Weigel, Detlef; Ho, William (2020), FT TFL1 bimolecular luciferase complementation assays, Dryad, Dataset, https://doi.org/10.5061/dryad.h9w0vt4fh

Abstract

CORRECTION

Ho, W.W.H., and Weigel, D. (2014). Structural Features Determining Flower-Promoting Activity of Arabidopsis FLOWERING LOCUS T. Plant Cell 26: 552-564. Published February 2014. DOI: https://doi.org/10.1105/tpc.113.115220.

During the assembly of Supplemental Figure 7 and Figure 8, six luciferase images of individual leaves were inadvertently duplicated. These images served to illustrate the strength of interactions between FT and TFL1 proteins and their mutant variants with GRF and TCP proteins, as measured qualitatively in split luciferase assays and in support of independent, quantitative yeast-two-hybrid experiments.

This Dryad data set includes the original photographs of bimolecular luciferase complementation assays after Agrobacterium tumefaciens mediated transient transformation of N. benthamiana leaves. The associated key links the constructs tested for bimolecular luciferase complementation with the photographs.

Methods

Bimolecular Luciferase Complementation Assays

Nicotiana benthamiana was transiently transformed as described (Voinnet et al., 2003), and samples were analyzed 4 days after inoculation (Gou et al., 2011). Before measurement, leaves only infiltrated with luciferin (negative control) and leaves infiltrated with a 35S:LUC construct and luciferin (positive control) were used for calibrating the Orca 2-BT cooled CCD camera (Hamamatsu Photonics, Hamamatsu, Japan).

Gou, J.Y., Felippes, F.F., Liu, C.J., Weigel, D., and Wang, J.W. (2011). Negative regulation of anthocyanin biosynthesis in Arabidopsis by a miR156-targeted SPL transcription factor. Plant Cell 23, 1512-1522.

Voinnet, O., Rivas, S., Mestre, P., and Baulcombe, D. (2003). An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J. 33, 949-956.

Funding

Croucher Foundation

Max-Planck-Gesellschaft