Coordinates activities of retrosplenial ensembles during resting-state encode spatial landmarks. Part 1 of 2
Chang, HaoRan et al. (2020), Coordinates activities of retrosplenial ensembles during resting-state encode spatial landmarks. Part 1 of 2, Dryad, Dataset, https://doi.org/10.5061/dryad.hhmgqnkd4
The brain likely uses off-line periods to consolidate recent memories. One hypothesis holds that the hippocampal output provides a unique, global linking or 'index' code for each memory, and that this code is stored in the cortex in association with locally encoded attributes of each memory. Activation of the index code is hypothesized to evoke coordinated memory trace reactivation thus facilitating consolidation. Retrosplenial cortex (RSC) is a major recipient of hippocampal outflow and we have described populations of neurons there with sparse and orthogonal coding characteristics that resemble hippocampal 'place' cells, and whose expression depends on an intact hippocampus. Using two-photon Ca2+ imaging, we recorded ensembles of neurons in the RSC during periods of immobility before and after active running on a familiar linear treadmill track. Synchronous bursting of distinct groups of neurons occurred during rest both prior to and after running. In the second rest epoch, these patterns were associated with the locations of tactile landmarks and reward. Complementing established views on the functions of the RSC, our findings indicate that the structure is involved with processing landmark information during rest.
This dataset includes imaging data collected from three Thy1-GCaMP6s transgenic mice in the Retrosplenial Cortex (RSC). Imaging data were acquired using a Thorlabs Bergamo II multiphoton microscope. Tissue was excited by a Ti:Sapphire laser (Coherent) tuned to an excitatory wavelength of 920 nm. Beam focusing and light collection were achieved by a 16X water immersion objective (Nikon, NA 0.8, 80-120 mW output power measured at the sample). Beam-scanning was conducted by Galvo-Resonant mirrors. Emitted fluorescent lights were detected by a GaAsP photomultiplier tube (Hamamatsu) and digitized to a resolution of 800x800 pixels at a sampling rate of 19 Hz. We collected an 835x835 um window over layers II-III of the agranular RSC at depths between 100 and 200 um (imaging windows centred at -1.8 to -2.5 mm AP, 0.5 mm ML).
Dataset divided into two DOIs due to large file size. See Related Resources for a link to the additional part.
The original size of the files was 560 GB. The files were archived using tar and compressed using pigz.
The folder structure is as follows: 1. animal ID, 2. date of recording, 3. session number. In folder 2 resides abf files generated from Clampex for individual recordings. Channel 1 contains frame pulses corresponding to the start and the end of rasterization of each individual recorded frame. Channels 2 and 3 correspond to outputs A and B respectively of the optical rotary encoder used in detecting the position of the animal over the treadmill belt. Finally, channel 5 contains the readout for the delivery of reward, which also serves to signal the start of a new lap. In folder 3 are raw imaging files along with xml files, which hold the experimental parameters, generated by the ThorImage software.
Part 1 of 2 files
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Natural Sciences and Engineering Research Council of Canada, Award: 1631465
Canadian Institutes of Health Research, Award: PJT 156040
Defense Advanced Research Projects Agency, Award: HR0011-18-2-0021